Biosensor

• US 8,679,310 B2
• Filed: 04/21/2011
• Issued: 03/25/2014
• Est. Priority Date: 04/22/2010
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Murase, Yosuke, Katsuki, Koji
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/091,325
• Publication Number:
  US 20110259741A1
• Time in Patent Office: 1,069 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.04
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/7203 for noise prevention, reduction or removal
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771

Abstract

A biosensor includes a first working electrode that a biocatalyst, which has a property that reacts on a specified ground substance, is disposed, a second working electrode that the biocatalyst, which the property is lost, is disposed, and at least one counter electrode for respectively applying a voltage to the first working electrode and the second working electrode.

Claims

1. A biosensor, comprising:

a first working electrode on which is disposed a first biocatalyst with the property that it reacts with a specific ground substance;

a second working electrode on which is disposed a second biocatalyst with the property that it also reacts with the specific ground substance; and

at least one counter electrode for applying a voltage to the first working electrode and the second working electrode,

wherein a rate of reaction of the first biocatalyst with the specific ground substance is different from a rate of reaction of the second biocatalyst with the specific ground substance, and

wherein the first biocatalyst and the second biocatalyst are enzymes of the same type.

2. A biosensor, comprising:

a first working electrode on which is disposed a first enzyme with the property that it reacts with a specific ground substance;

a second working electrode on which is disposed a second enzyme with the property that it reacts with the specific ground substance; and

at least one counter electrode for applying a voltage to the first working electrode and the second working electrode,

wherein the second enzyme is obtained by genetic modification of the first enzyme so that a rate of reaction of the second enzyme with the specific ground substance is different from a rate of reaction of the first enzyme with the specific ground substance.

3. A biosensor in combination with a monitoring apparatus, wherein the biosensor comprises:

a first working electrode on which is disposed a first biocatalyst with the property that it reacts with a specific ground substance;

a second working electrode on which is disposed a second biocatalyst with the property that it also reacts with the specific ground substance; and

at least one counter electrode for applying a voltage to the first working electrode and the second working electrode,

wherein a reaction area on the first working electrode is different from a reaction area on the second working electrode, and

wherein the monitoring apparatus comprises;

a first detection unit which detects a first signal value from the first working electrode obtained by applying a voltage between the first working electrode and one of the counter electrodes;

a second detection unit which detects a second signal value from the second working electrode obtained by applying a voltage between the second working electrode and one of the counter electrodes;

a correction unit which corrects a concentration of the specific ground substance that is calculated from the first signal value by the second signal value; and

an output unit which outputs the corrected concentration of the specific ground substance,

wherein the first working electrode is used to detect the first signal value to calculate a concentration of the specific ground substance and the second working electrode is used to detect the second signal value to correct the concentration of the specific ground substance calculated from the first signal value,

wherein the corrected concentration of the specific ground substance is obtained based on the formula;

x(t)=(y(t)−

y′

(t))/(a−

b)

wherein in the formula, a first signal value y(t) at a certain point of time t is expressed by a linear function

y(t)=ax(t)+c,

where a is a calibration curve coefficient corresponding to the first biocatalyst, x is a concentration of the specific ground substance and c is a signal component based on a substance excluding the specific ground substance in a body fluid;

a second signal y′

(t) at a certain point of time t is expressed by a linear function

y′

(t)=bx(t)+c,

where b is a calibration curve coefficient corresponding to the second biocatalyst, x is the concentration of the specific ground substance and c is the signal component; and

a value of the signal component c in the first signal value y(t) is equal to a value of the signal component c of the second signal value y′

(t).

4. A biosensor in combination with a monitoring apparatus, wherein the biosensor comprises:

a first working electrode on which is disposed a first biocatalyst with the property that it reacts with a specific ground substance;

a second working electrode on which is disposed a second biocatalyst with the property that it also reacts with the specific ground substance; and

at least one counter electrode for applying a voltage to the first working electrode and the second working electrode,

wherein a total reaction activity of the first working electrode is different from a total reaction activity of the second working electrode, and

wherein the monitoring apparatus comprises;

a first detection unit which detects a first signal value from the first working electrode obtained by applying a voltage between the first working electrode and one of the counter electrodes;

a second detection unit which detects a second signal value from the second working electrode obtained by applying a voltage between the second working electrode and one of the counter electrodes; and

a correction unit which corrects a concentration of the specific ground substance that is calculated from the first signal value by the second signal value; and

an output unit which outputs the corrected concentration of the specific ground substance,

wherein the first working electrode is used to detect the first signal value to calculate a concentration of the specific ground substance and the second working electrode is used to detect the second signal value to correct the concentration of the specific ground substance calculated from the first signal value,

wherein the corrected concentration of the specific ground substance is obtained based on the formula

x(t)=(y(t)−

y′

(t))/(a−

b)

wherein in the formula, a first signal value y(t) at a certain point of time t is expressed by a linear function y(t)=ax(t)+c, where a is a calibration curve coefficient corresponding to the first biocatalyst, x is a concentration of the specific ground substance and c is a signal component based on a substance excluding the specific ground substance in a body fluid;

a second signal y′

(t) at a certain point of time t is expressed by a linear function

y′

(t)=bx(t)+c,

where b is a calibration curve coefficient corresponding to the second biocatalyst, x is the concentration of the specific ground substance and c is the signal component; and

a value of the signal component c in the first signal value y(t) is equal to a value of the signal component c of the second signal value y′

(t), and

wherein the first biocatalystand the second biocatalyst are enzymes of the same type.

5. A monitoring apparatus, comprising:

a first detection unit which detects a first signal value from a first working electrode obtained by applying a voltage between the first working electrode and a counter electrode, wherein disposed on the first working electrode is a first biocatalyst with the property that it reacts with a specific ground substance;

a second detection unit which detects a second signal value from a second working electrode obtained by applying a voltage between the second working electrode and the counter electrode, wherein disposed on the second working electrode is a second biocatalyst with the property that it also reacts with the specific ground substance and has a rate of reaction different from that of the first biocatalyst;

a correction unit which corrects a concentration of the specific ground substance that is calculated from the first signal value by the second signal value; and

an output unit which outputs the corrected concentration of the specific ground substance.

6. The monitoring apparatus according to claim 5, wherein the correction unit obtains a concentration x(t) of said specific ground substance based on the formula

x(t)=(y(t)−

y′

(t))/(a−

b)

wherein in the formula, the first signal value y(t) at a certain point of time t is expressed by a linear function

y(t)=ax(t)+c,

where a is a calibration curve coefficient corresponding to the first biocatalyst, x is a concentration of the specific ground substance and c is a signal component based on a substance excluding the specific ground substance in a body fluid;

the second signal y′

(t) at the certain point of time t is expressed by a linear function

y′

(t)=bx(t)+c,

where b is a calibration curve coefficient corresponding to the second biocatalyst, x is the concentration of the specific ground substance and c is the signal component; and

a value of the signal component c in the first signal value y(t) is equal to a value of the signal component c of the second signal value y′

(t).