Methods and compositions for tissue regeneration
First Claim
1. A cell preparation comprising mitotically inactive allogeneic cells that are capable of secreting one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
- , angiopoietin, EGF, IL-1β
, IL-6, IL-8, TGFα
, and TNFα
, wherein the cells are keratinocytes or fibroblasts, or mixtures thereof, and wherein the cell preparation is comprised in a buffered solution, a wound dressing, or an extracellular matrix or matrix material.
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Accused Products
Abstract
A kit is disclosed that includes a first component comprising alginate, wherein the first component is comprised in a first sterile vial, and a second component comprising cells comprising keratinocytes or fibroblasts, or mixtures thereof, that secrete one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ, angiopoietin, EGF, IL-Iβ, IL-6, IL-8, TGFα, and TNFα, wherein the cells are allogeneic and mitotically inactive, a buffered solution, and human serum albumin or a cryoprotectant, wherein the second component is comprised in a second sterile vial.
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Citations
34 Claims
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1. A cell preparation comprising mitotically inactive allogeneic cells that are capable of secreting one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
- , angiopoietin, EGF, IL-1β
, IL-6, IL-8, TGFα
, and TNFα
, wherein the cells are keratinocytes or fibroblasts, or mixtures thereof, and wherein the cell preparation is comprised in a buffered solution, a wound dressing, or an extracellular matrix or matrix material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- , angiopoietin, EGF, IL-1β
-
17. A cell preparation comprising mitotically inactive allogeneic cells that are capable of secreting one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
- , angiopoietin, EGF, IL-Iβ
, IL-6, IL-8, TGFα
, and TNFα
, wherein the cells are keratinocytes or fibroblasts, or mixtures thereof, and wherein the cell preparation is in the form of a paste or spray. - View Dependent Claims (18, 19, 20, 21, 22, 23)
- , angiopoietin, EGF, IL-Iβ
-
24. A cell preparation comprising mitotically inactive allogeneic cells that are capable of secreting one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
- , angiopoietin, EGF, IL-Iβ
, IL-6, IL-8, TGFα
, and TNFα
, wherein the cells are a mixture of keratinocytes and fibroblasts and the ratio of keratinocytes to fibroblasts is 1;
9. - View Dependent Claims (25, 26, 27, 28)
- , angiopoietin, EGF, IL-Iβ
-
29. A cell preparation comprising mitotically inactive allogeneic cells that are capable of secreting one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
- , angiopoietin, EGF, IL-Iβ
, IL-6, IL-8, TGFα
, and TNFα
, wherein the cells are keratinocytes or fibroblasts, or mixtures thereof, and wherein the cell preparation further comprises a cryoprotectant. - View Dependent Claims (30, 31, 32, 33, 34)
- , angiopoietin, EGF, IL-Iβ
Specification