Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
First Claim
Patent Images
1. An isolated polynucleotide encoding a variant polypeptide of SEQ ID NO:
- 1, the variant polypeptide having fluorescence, having reduced aggregation relative to SEQ ID NO;
1, having a sequence identity of 90% or more with SEQ ID NO;
1 or SEQ ID NO;
5, and comprising a proline residue at position 10 of SEQ ID NO;
1 or SEQ ID NO;
5.
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Abstract
Polynucleotides encoding variant polypeptides of DsRed are provided herein. The DsRed variants have increased bacterial expression, reduced aggregation, increased solubility, shifted emission spectra or increased brightness relative to a wild-type DsRed.
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Citations
21 Claims
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1. An isolated polynucleotide encoding a variant polypeptide of SEQ ID NO:
- 1, the variant polypeptide having fluorescence, having reduced aggregation relative to SEQ ID NO;
1, having a sequence identity of 90% or more with SEQ ID NO;
1 or SEQ ID NO;
5, and comprising a proline residue at position 10 of SEQ ID NO;
1 or SEQ ID NO;
5. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
- 1, the variant polypeptide having fluorescence, having reduced aggregation relative to SEQ ID NO;
Specification