Kits pertaining to analyte determination

• US 8,679,773 B2
• Filed: 01/16/2007
• Issued: 03/25/2014
• Est. Priority Date: 01/30/2003
• Status: Active Grant

• Current Assignee: DH Technologies Development Pte. Ltd. (Danaher Corporation)
• Original Assignee: DH Technologies Development Pte. Ltd. (Danaher Corporation)
• Inventors: Pappin, Darryl J. C., Bartlett-Jones, Michael
• Primary Examiner(s): Grazier, Nyeemah A
• Application Number:
  US11/623,510
• Publication Number:
  US 20110045516A1
• Time in Patent Office: 2,625 Days
• Field of Search: None
• US Class Current: 435/18
• CPC Class Codes: A61K 51/04 Organic compounds
• CPC Class Codes: C07D 207/46 with hetero atoms directly attached to the ring nitrogen atom
• CPC Class Codes: C07D 401/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: G01N 2458/15 Non-radioactive isotope labels, e.g. for detection by mass spectrometry
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: Y10T 436/142222 Hetero-O [e.g., ascorbic acid, etc.]
• CPC Class Codes: Y10T 436/145555 Hetero-N
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Claims

1. A kit comprising:

a) a set of two or more isobaric and/or isomeric reagents for the labeling of analytes, wherein all reagents of the set comprise the formula;

2. The kit of claim 1, wherein the kit comprises a proteolytic enzyme.

3. The kit of claim 1, wherein the active ester is an N-hydroxysuccinimide ester.

4. The kit of claim 1, wherein each reagent of the set is independently linked to a solid support through a cleavable linker.

5. The kit of claim 1, wherein all reagents of the set are isomeric.

6. The kit of claim 1, wherein all reagents of the set are isobaric.

7. The kit of claim 1, wherein the set comprises one or more of the following four reagents:

8. The kit of claim 1, wherein the set comprises one or more of the following four reagents:

9. The kit of claim 1, wherein the set comprises one or more of the following four support bound reagents:

10. The kit of claim 1, wherein each reagent of the set is isotopically enriched with one or more heavy atom isotopes.

11. The kit of claim 10, wherein each heavy atom isotope is present in at least 80% isotopic purity.

12. The kit of claim 10, wherein each heavy atom isotope is present in at least 93% isotopic purity.

13. The kit of claim 10, wherein each heavy atom isotope is present in at least 96% isotopic purity.