Apparatus and methods for analyte measurement and immunoassay
First Claim
1. A method of measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit, said method comprising:
- contacting said sensor with a liquid sample containing said analyte;
contacting said sensor with an enzyme-labeled antibody capable of binding said analyte, whereby a complex of immobilized antibody, analyte and labeled antibody is formed;
contacting said sensor with a solution comprising a substrate for said enzyme and at least one air segment to remove unbound analyte and labeled antibody from a region of the sensor;
removing substantially all said solution from said sensor while retaining a portion of said solution over said electrode, said counter/reference electrode, and a contiguous portion of a wall of the conduit connecting said electrodes; and
detecting a product of a reaction between said enzyme-labeled antibody and said substrate using said sensor, whereby the amount of said analyte in the liquid sample is measured.
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Abstract
The present invention relates to methods for measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit. A solution comprising a substrate for said enzyme and at least one air segment contacts the sensor to remove unbound analyte and labeled antibody from a region of the sensor. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.
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Citations
11 Claims
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1. A method of measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit, said method comprising:
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contacting said sensor with a liquid sample containing said analyte; contacting said sensor with an enzyme-labeled antibody capable of binding said analyte, whereby a complex of immobilized antibody, analyte and labeled antibody is formed; contacting said sensor with a solution comprising a substrate for said enzyme and at least one air segment to remove unbound analyte and labeled antibody from a region of the sensor; removing substantially all said solution from said sensor while retaining a portion of said solution over said electrode, said counter/reference electrode, and a contiguous portion of a wall of the conduit connecting said electrodes; and detecting a product of a reaction between said enzyme-labeled antibody and said substrate using said sensor, whereby the amount of said analyte in the liquid sample is measured. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification