Microarray system

• US 8,680,025 B2
• Filed: 05/09/2008
• Issued: 03/25/2014
• Est. Priority Date: 05/09/2008
• Status: Active Grant

• Current Assignee: Akonni Biosystems, Inc.
• Original Assignee: Akonni Biosystems, Inc.
• Inventors: Cooney, Christopher G.
• Primary Examiner(s): BOESEN, CHRISTIAN C
• Application Number:
  US12/149,865
• Publication Number:
  US 20090280997A1
• Time in Patent Office: 2,146 Days
• Field of Search: None
• US Class Current: 506/39
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides

Abstract

A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.

Claims

1. A microarray system, comprising:

a microarray formed on a planar substrate;

an incubation chamber comprising an enclosed space formed around said microarray, wherein said incubation chamber is connected to one inlet and one outlet channel;

a one-way valve for loading a liquid sample into said incubation chamber, wherein said one-way valve is connected to said incubation chamber through said inlet channel; and

a waste chamber in fluid communication with said incubation chamber through said outlet channel,

wherein said incubation chamber comprises a plurality of interior surfaces including a bottom surface on which said microarray is formed and a top surface that faces said microarray,

wherein said top surface is a hydrophilic surface and said bottom surface is a hydrophilic surface and wherein said top and bottom hydrophilic surfaces overcome the surface tension of a water-based liquid and allows complete filling of said incubation chamber with said water-based liquid,

wherein said outlet channel comprises an inlet section, a funnel shape connecting section, and an outlet section, wherein said inlet section has a diameter that is larger than a diameter of said outlet section, and

wherein said waste chamber has a volume that is larger than a volume of said incubation chamber and comprises an absorbent capable of wicking liquid from said incubation chamber,

wherein said incubation chamber is formed by placing a gasket around said microarray and covering said gasket with a hydrophilic tape or a hydrophilic film, wherein said top surface consists of the hydrophilic tape or the hydrophilic film.

2. The microarray system of claim 1, wherein said hydrophilic tape or hydrophilic film is transparent.

3. The microarray system of claim 1, wherein said bottom surface comprises impregnated chemicals that lyses cell membranes.

4. The microarray system of claim 3, wherein said bottom surface comprises a hydrophilic matrix that retains nucleic acid from lysed cells.

5. The microarray system of claim 1, wherein said one-way valve is a check valve.

6. The microarray system of claim 1, wherein said one-way valve is a dome valve.

7. The microarray system of claim 1, wherein said absorbent comprises cellulose.

8. The microarray system of claim 1, wherein said absorbent is placed at a distance from said outlet channel to control wicking rate.

9. The microarray system of claim 1, wherein said waste chamber is vented to atmosphere through a venting channel.

10. The microarray system of claim 1, wherein said substrate is glass.

11. The microarray system of claim 1, wherein said substrate is plastic.

12. The microarray system of claim 1, wherein said microarray is an oligonucleotide array.

13. The microarray system of claim 1, wherein said microarray is a protein array.

14. The microarray system of claim 13, wherein said protein array is an antibody array.

15. The microarray system of claim 1, wherein said microarray is formed by a gel spot printing method.