Apparatus and method for the purification of biomolecules

• US 8,685,322 B2
• Filed: 11/13/2008
• Issued: 04/01/2014
• Est. Priority Date: 11/13/2007
• Status: Active Grant

• Current Assignee: Stratec Es
• Original Assignee: Stratec Biomedical AG
• Inventors: Griebel, Ralf, Joos, Hans, Bendzko, Peter
• Primary Examiner(s): HIXSON, CHRISTOPHER
• Application Number:
  US12/270,621
• Publication Number:
  US 20090176308A1
• Time in Patent Office: 1,965 Days
• Field of Search: None
• US Class Current: 422/67
• CPC Class Codes: B01F 31/441 performing a rectilinear reciprocating movement
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

The invention comprises an apparatus 10 and a method 100 for purifying biomolecules 28. Magnetizable particles 35 and a group of magnetizable pins 40 are used for binding biomolecules 28 to the magnetizable particles 35 using a binding buffer 30b, and for transferring the magnetizable particles 35 forming a particle-biomolecule complex 36. The apparatus 10 comprises an incubation unit 60 that allows for an upscaling of a maximum volume of a sample material. Furthermore the apparatus 10 comprises counter magnets 50 which are arranged on cavities 20. Solutions 30 comprising the biomolecules 28 and the magnetizable particles 35 are disposed in the cavities. The use of counter magnets 50 improves the quality of an eluate. Furthermore the invention comprises a system 800 and a method 900 for a diagnostically purifying of biomolecules 28. The method 900 further comprises a controlling of method steps and a selection of substances and reagents (for example the magnetizable particles 35) based on information automatically collected from, for example, the samples and/or derived from used substances and reagents. The system 800 fulfills requirements of medical diagnostics.

Claims

1. A system for diagnostically purifying biomolecules comprising:

a plurality of cavities for accommodating solutions, the plurality of cavities comprising at least one sample cavity for a sample material, wherein the sample material comprises the biomolecules and wherein the sample cavity bears an unambiguous identification, at least one yield cavity, and at least one of a lysis cavity, one or more washing cavities and an elution cavity,

at least one movable and magnetizable pin arranged in such a fashion that the movable and magnetizable pin can be inserted in at least one of the lysis cavity, in the one or more washing cavities and in the elution cavity,

a control unit for controlling the system,

magnetizable particles to which the biomolecules are attachable to form a particle-biomolecule complex

wherein the system yields an eluate with the diagnostically purified biomolecules from the sample material by transporting the particle-biomolecule complex with the at least one movable and magnetizable pin to at least the elution cavity wherein the biomolecules are desorbed from the magnetizable particles, and

a coding detection and transfer unit for the unambiguous identification of a coding relating to the content of the sample material in the at least one sample cavity and for reallocating the coding to the result in the at least one yield cavity.

2. The system according to claim 1 furthermore comprising:

a loading bay for accommodating substances, wherein the substances are at least one of the sample material, a buffer solution or the magnetizable particles,

a reading module for automatically identifying the substances and the sample material upon accommodating in the loading bay,

an incubation unit for heating, thermostating and incubating the samples in the lysis cavity,

a pipetting unit for transferring the substances,

a receptacle.

3. The system according to claim 1, furthermore comprising:

apparatus for improving the quality of the eluate before removing by pipetting adapted to keep the magnetizable particles away from a tip of the pipetting device.

4. The system according to claim 1, furthermore comprising:

sensors for recording and logging a plurality of parameters using a documentation unit.

5. The system according to claims 1, furthermore comprising:

a plurality of parameter controls for controlling the plurality of parameters using the control unit.

6. The system according to claim 2, wherein the control unit is adapted to check a consistency of the substances disposed in the loading bay based on the substances in the loading bay.

7. The system according to claim 6, wherein the control unit is adapted to check, if the consistency of the substances disposed in the loading bay is correct based on a composition of a kit.

8. The system according to claim 6, wherein the control unit is adapted to output a message in the case that the consistency of the substances in the loading bay is not correct.

9. The system according to claim 6, wherein the control unit is adapted to prevent an opening of the loading bay during the controlling of the system, in case that the consistency of the substances disposed in the loading bay is correct.

10. The system according to claim 2, wherein the system is adapted to determine a substance amount of required buffer solutions in the loading bay based on the identified substance in the loading bay.

11. The system according to claim 2, wherein the system is adapted to determine a substance amount of the required magnetizable particles based on the identified substance in the loading bay.

12. The system according to claim 2, wherein the system is adapted such that the system determines a number and a sequence of process steps of a method for diagnostically purifying biomolecules based on the identified substance in the loading bay.

13. The system according to claim 2, wherein the system is adapted to determine an appropriate incubation volume for incubating based on the identified substance in the loading bay.

14. The system according to claim 13, wherein the system is adapted to determine a suitable multiwell plate for incubating based on the appropriate incubation volume.

15. The system according to claims 1, wherein the system is adapted to automatically select, request and use at least one suitable cavity from the group consisting of lysis cavity, incubator cavity, work plate, cavity of the work plate, yield plate.