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Amnion-derived cell compositions, methods of making and uses thereof

  • US 8,685,390 B2
  • Filed: 10/03/2012
  • Issued: 04/01/2014
  • Est. Priority Date: 03/31/2005
  • Status: Active Grant
First Claim
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1. A method for accelerating wound healing of a chronic, infected wound as compared to the healing rate of a non-treated chronic, infected wound in a patient in need thereof comprising treating the chronic, infected wound by topically administering to the patient'"'"'s chronic, infected wound about 0.25-1.0×

  • 106 cultured amnion-derived epithelial cells, wherein the cultured amnion-derived epithelial cells are made by the method comprising the steps ofa) obtaining a placenta and isolating an amnion from the placenta,b) enzymatically releasing amnion-derived epithelial cells from the amnion,c) collecting the released amnion-derived epithelial cells, andd) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF.

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