Continuous multi-microencapsulation process for improving the stability and storage life of biologically active ingredients

• US 8,685,446 B2
• Filed: 09/17/2010
• Issued: 04/01/2014
• Est. Priority Date: 12/18/2003
• Status: Active Grant

• Current Assignee: Chemische Fabrik Budenheim KG (Dr August Oetker KG)
• Original Assignee: GAT Food Essentials GmbH
• Inventors: Casana-Giner, Victor, Gimeno-Sierra, Miguel, Gimeno-Sierra, Barbara, Moser, Martha
• Primary Examiner(s): Hartley, Michael G
• Assistant Examiner(s): YOUNG, MICAH PAUL
• Application Number:
  US12/885,165
• Publication Number:
  US 20110064778A1
• Time in Patent Office: 1,292 Days
• Field of Search: 424464-489, 424/451, 424/452, 424/458
• US Class Current: 424/463
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: B01J 13/18 In situ polymerisation with all reactants being present in the same phase

Abstract

The invention relates to microcapsules, and a continuous micro-encapsulation water-in-oil-in-water microencapsulation process through in situ and interfacial polymerization of the emulsion. The formulation comprises a continuous water phase having a dispersion of microcapsules which contain oil drops and wherein the inside of each oil phase drop—containing optionally oil-soluble materials—there is a dispersion of water, or aqueous extract or water dispersible material or water soluble material. The oil drops are encapsulated with a polymerisable material of natural origin. Such microcapsules are appropriated for spray-dry processes, to be used as dry powder, lyophilised, self-emulsifiable powder, gel, cream and any liquid form. The active compounds included in the microcapsules are beneficial to the health and other biological purposes. Such formulations are appropriate to be incorporated in any class of food, especially for the production of nutraceuticals, as well as cosmetic products (such as rejuvenescence creams, anti-wrinkle creams, gels, bath and shower consumable products and sprays). The preparations are adequate to stabilise compounds added to the food, media for cultivating microbes and nutraceuticals, especially those which are easily degradable or oxidizable.

Claims

1. Microcapsules, characterized in that:

(a) each of the microcapsules having a wall containing an inner content of the microcapsules,

(b) the wall of each of the microcapsules is formed by at least two polymerized hydrocolloids,

(c) the inner content of the microcapsules consists in a first emulsion of water in oil and first inner microcapsules suspended in the first emulsion,

(d) the average particle size of each of the microcapsules is 1-10 μ

m,

(e) at least one biologically active ingredient is present in at least a discontinuous oil phase and/or in a discontinuous water phase in the microcapsules,

(f) the first inner microcapsules each having a wall formed by the at least two polymerized hydrocolloids, and

(g) the inner content of the first inner microcapsules each comprising a second emulsion of water in oil.

2. Microcapsules according to claim 1, characterized in that the microcapsules contain at least one biologically active compound originated by the group of organisms:

Medicago sativa, Pimenal officinalis, Hibiscus abelmoschus, Angelica archangelica, Galipea officinalis, Pimpinella anisum, Ferula foetida, Ferula asafetida, Melissa officinalis, Myroxylon pereirae, Ocimum basilicum, Pimenta acris, Citrus aurantium bergamia, Prunus amygdalus, Citrus aurantium, Citrus aurantium amara, Piper nigrum, Prunus spinosa, Aniba rosaeodora, Camelia oleifera, Camelia sinensis, Carum carvi, Elettaria cardamomum, Ceratonia siliqua, Daucus carota, Dacus carota sativa, Cascarilla, Apium graveolens, Anthemis nobilis, Matricaria chamomilla, Anthemis nobilis, Anthriscus cerefolium, Cichorium intybus, Cinnamomum spp., Cinnamomum zeylanicum, Cymbopogon nardus, Salvia sclarea, Trifolium pratense, Theobroma cacao, Coffea arabica, Coriandrium sativum, Cuminum cyminum, Taraxacum officinale, Sambucus nigra, Edelweiss, Helichrysum italicum, Foeniculum vulgare, Trigonella foenumgraecum, Arabidopsis spp., Zingiber officinale, Citrus grandis, Psidium guajava, Humulus lupus, Marrubium vulgare, Monarda punctata, Hyssopus officinals, Jasminum officinale, Jasminum grandiflorum, Juniperusspp. Juniperus comunis, Eucaliptus officinalis, Cola acuminata, Laurus nobilis, Lavandula spp.,Lavandula hybrida, Taxus baccata, Citrus medica limonum, Myristica fragans, Marjorana hortensis, Thymus spp., Thymus officinalis, Thymus mastichina, Ilex paraguarensis, Chamomilla recutita, Saccharum officinarum, Myristica fragans, Allium cepa, Citrus aurantium dulcis, Carum petroselinum, Mentha pulegium, Mentha piperita, Pimenta officinalis, Chimaphila umbellate, Punica granatum, Pelargonium spp., Pelargonium graveolens, Rosmarinus officinalis, Crocus sativus, Salvia spp., Salvia officinalis, Mentha spicata, Mentha viridis, Satureia hortensis, Satureja hortensis, Origanum majorana, Tamarindus indica, Citrus reticulata, Artemisia dracunculus, Thea sinensis, Thymus vulgaris, Polianthes tuberosa, Curcuma longa, Prunus serotina, Thymus serpillum, Satureja Montana, Cananga odorata, Curcuma zedoaria, Plantago major, Adansonia digitata, Ananas comosus, Artocarpus altilis, Carica papaya, Lycopersicon esculentum, Cephalophus spp., Vaccinium myrtillus, Thymus aragonensis, Thymus spp., Citrus aurantiifolia, Citrus paradisi, Cucumis melo, Cucurbita spp., Vitis spp., Vitis vinifera, Mangifera indica, Lamiaceae (Coleus, Hedeoma, Hyptis, Leonurus, Leucas, Lycopus, Marrubium, Mentha, Monarda, Perilla, Prunella, Salvia, Stachys, Teucrium, Thymus), Cannabis spp., Digitalis lanata, Adonis vernalis, Aesculus hippocastanum, Frazinus rhychophylla, Agrimonia supatoria, Rauvolfia sepentina, Andrographis paniculata, Areca catechu, Atropa belladonna, Berberis vulgaris, Ardisia japonica, Betula alba, Ananas comosus, Camellia sinensis, Cinnamomum camphora, Camptotheca acuminata, Potentilla fragarioides, Erythroxylum coca, Papaver somniferum, Colchicum autumnale, Claviceps purpurea, Digitalis purpurea, Digitalis lanata, Glaucium flavum, Gossypium spp., Hyoscyamus niger, Camptotheca acuminata, Piper methysticum, Lobelia inflata, Crotalaria sessiliflora, Nicotiana tabacum, Physostigma venenosum, Ephedra sinica, Cinchona ledgeriana, Rhododendron molle, Datura spp., Taxus brevifolia, Strychnos nux-vomica, Stevia rebaudiana, Valeriana officinalis, Pausinystalia yohimbe, Ephedra spp., Crataegus oxyacantha, Hamamelis virginiana, Hydrastis Canadensis, Hypericum perforatum, Potentilla erectra, Ledum palustre, Salvia officinalis, Chamomilla recutita, Arctostaphylos uva, Eucommia ulmoides, Mytilus galloprovincialis, Diplazium esculentum, Manihot utillissima, Sauropous androgynus, Terminalia arjuna, Iberis amara, Crataegus spp., Arbutus unedo, Cynara scolymus, Amaranthus caudatus, Alchornea laxiflora, Alpinia officinarum, Xanthophyllomyces dendrorhous, Crataegus monogyna, Taxus yunnanensis, Bacopa monniera, Cistus albidus, Ocimum basilicum, Rosmarinus officinalis, Thymus vulgaris, Bixa orellana, Centella asiatica, Urtica dioica, Agrocybe aegerita, Crataegus laevigata, Satureja hortensis, Crocus sativus, Coccinia indica, Brugia malayi, Rubus spp., Silybum marianum, Cannabis sativa, Hypericum perforatum, Rhus coriaria, Olea europaea, Cyclopia intermedia, Ginkgo biloba, Lentinus lepideus, Pseudomonas putida, Sargassum micracanthum, Pinus radiata, Pinus spp., Phaseoulus mungo, Cicer arietinum, Vigna sinensis, Phaseolus aureus, Dolichos lablab, Cajanus cajan, Vicia faba, Dolichos biflorus, Phaseolus lunatus, Phaseolus aconitifolius, Pisum sativum, Psophocarpus tetragonolobus, Arachis hypoagea, Brassica spp., Brassica campestris, Brassica napus, Valeriana officinalis, Echinacea purpurea, Echinacea pallida, Echinacea angustifolia, Glcyrrhiza glabra, Seronea repens, Vaccinium macrocarpon, Tancetum parthenuum, Tancetum parthenuum, Vaccinium macrocarpon, cereals, seed fruits, silvestre bays, leguminosae, green tea, black tea and microorganisms able to produce long-chained unsaturated fatty acids.

3. Microcapsules according to claim 1, characterized in that the microcapsules contain at least one of the biologically active materials selected from (A) and/or (B) in all their stereochemical and isomeric variations:

4. Microcapsules according to claim 1 characterized in that they contain at least one unsaturated long-chain fatty acid of at least 6 carbon atoms, in any isomeric and/or stereochemical configuration, as well as any esters, ethers, glycerides, phospholipids, and sphingolipids derivatives thereof.

5. Microcapsules according to claim 1 characterized in that they contain unsaturated fatty acids with a carbon chain of at least 6 carbon atoms that come from the following groups of natural sources or from genetically modified organisms of the following natural sources:

(a) vegetable origin;

Boraginaceae, (Borago spp., Borago officinalis);

Linaceae (Linum usitatissimum, Linum arvense, Linum sativum);

Onograceae (Oenothera Biennis);

Grossulariaceae\ (Ribes nigrum), Zea Mais, Gossypium hirsutum, Carthamus tinctorius, Glycine max. Boraginaceae, (Borago spp., Borago officinalis), Linaceae (Linum usitatissimum, Linum arvense, Linum sativum), Onograceae (Oenothera biennis), Grossulariaceae (Ribes nigrum), Zea Mais, Gossypium hirsutum, Carthamus tinctorius, Glycine max;

(b) algae origin;

Graciliariceae (Gracilaria spp.), Gigartinaceae (Iridaea spp.), Kallymeniaceae (Callopyllis varicgata), Durvillaceae (Durvillaea antartica), Solieriaceae (Euchema cottoni), Gelidiaceae (Gelidium spp,), Lossoniaceae (Lesonia nigrescens), Gigantinaceae (Gigartina spp.), Lessoniaceae (Macrocystis spp.), Bangiaceae (Porphyra spp.), Crypthecodinium spp.;

(c) animal origin;

Engaulidae (Lycengraulis olidus), Clupeidae (Sardina pilchardus), Scomberesocidae (Scomberesox saurus scombroides), Berycidae (Beryx splendens), Engraulidae (Engraulis ringens), Ophichthyidae (Ophichthus spp.), Serranidae (Hemilutjanus macrophthalmus), Scombridae (Thunnus spp., en especial, Thunnus albacares, Thunnus alalunga, Thunnus obesus, Sarda spp., Sarda chiliensis, Scomber japonicus peruanus)), Sciaenidae (Cynoscion analis), Carcharhinidae (Prionace glauca), Normanichthyidae (Normanichthys crockeri), Percichthyidae (Polyprion oxygeneios), Nototheniidae (Dissostichus eleginoides), Apogonidae (Epigonus crassicaudus), Branchiostegidae (Prolatilus jugularis), Normanichthyidae (Normanichthys crockeri), Percichthyidae (Polyprion oxygeneios), Nototheniidae (Bacalao de profundidad), Apogonidae (Epigonus crassicaudus), Branchiostegidae (Prolatilus jugularis), Cheilodactylidae (Cheilodactylus gavi), Gadidae (Salilota australis), Pomadasvidae, Scorpaenidae, Serranidae, Cyprinidae, Monacanthidae, Centrolophidae, Ophidiidae, Coryphaenidae, Channichthydae, Sciaenidae, Aplodactylidae, Carangidae (Trachurus symetricus murphyi), Bothidae (Paralichthys microps), Mugilidae, Priacathidae, Merlucciidae (Merluccius gayi gayi, Merluccius australis), Macruronidae (Macruronus magellanicus), Gadidae (Micromesistius australis), Girellidae, Trachichthvidae, Carangidae, Kvphosidae, Callorhvnchidae, Labridae, Macrouridae, Atherinidae, Gobiesocidae, Alopiidae, Galaxiidae, Rajidae, Bramidae, Carangidae, Nototheniidae, Scianidae, Mugiloididae, Salmonidae (Salmo spp., Salmo salar, Oncorhynchus spp., Oncorhynchus kisutch, Oncorhynchus mykiss, Oncorhynchus tshawytscha), Clupeidae (Sardinops spp., Sardinops sagax, Clupea bentincki), Pomadasvidae, Gempvlidae, Lamnidae (Isurus spp., Isurus oxyrinchus), Triakidae, Clinidae, Scophthalmidae, and more preferably Atlantic mackerel, Engraulis encrasicholus, Pomatomus saltatrix, Sarda sarda, Sardina pilchardus, Brevoortia tyrannus, Brevoortia patronus, Chloroscombrus chrysurus, Auxis thazard, Scomber scombrus, Scomber japonicus, Alosa aestivalis, Clupea harengus, Etrumeus teres, Argentina silus, Ictalurus punctatus; and

(d) microbial origin;

Saccharomices cerevisiae, Escherichia coli, Schizochytrium spp., Thraustochytrium aurcum, Thraustochytrium roscum, Thraustochytrium striatum, Mortiriella spp., Phytium spp., Aspergillus spp. Aspergillus nidulans, Aspergillus sydowi, Fusarium spp., Fusarium equiseti, Fusarium oxysporum.

6. Microcapsules produced according to claim 1, characterized in that the microcapsules are stable and the microcapsule'"'"'s wall does not open at pH higher than 3.5.

7. Microcapsules according to claim 1 characterized in that they contain in the oil phase at least one compound selected from:

fish oil, flax oil, omega-3 and/or omega-6 and/or omega-9 fatty acids, conjugated linoleic acids, terpenoids, carotenoids, tocotrienols, flavonoids, hormones, antioxidants.

8. Microcapsules according to claim 1 characterized in that they contain in the water phase at least one compound selected from:

polyphenols, proantocyanidins, anthocyans, alpha-lipoic acid, coenzyme Q-10 aminoacids, peptides, enzymes, enzyme inhibitors, minerals, oligoelements, green tea extracts, rosemary extracts, Thymus spp. extracts, raspberry extracts, Mentha piperita extracts, iron, selenium, magnesium, antioxidants, hormones, probiotic bacteria or fungi or yeasts.

9. Microcapsules according to claim 1 characterized in that they contain at least one biologically active material at least a biologically active material selected from the groups;

(a) flavonoids, anthocyianidins, pro-anthocyanidins, oligomeric proanthocyanidins, isoflavones, chalcones, catechin, epihatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate, eriodtrin, narirutin, rutin, naringin, myricitrin, hesperidin, myricetin, eriodictyol, fisetin, quercetin, naringenin, luteolin, hesperitin, kaempferol, isorhamnetin, apigenin, rhamnetin, galangin, quercitrin, diosmetin, taxifolin, galandin, biochanin A, genistein, eriodictyol, chrysin, hydroxytyrosol, oleuropein, glabridine, licochalcone, daidzein, matairesinol, secoisolariciresinol, enterodiol, enterolactone, equol, desmethylangolensin, luteoferol, luteolinidin, apiferol, apigenidin, leucocyanidin, taxifolin, pelargonidin;

(b) phenolic acids in general and esters, glycosides, rutinosides and amines thereof, gallic, sinapic, syringic, caffeic, chlorogenic, ferulic, procatechuic, vanillic, hydroxycinnamic, and coumaric acids, guaiacol, cresol, 4-ethylphenol, 4-vinylguaicol, eugenol, tannins, ellagiotannins, gallotannins;

(c) esctructurally combined amides comprising hydroxycinnamic acids and anthranilic acids (avenanthramides), avenasterol, long-chain fatty acids or alcohols, indoleamines, melatonin, inulin, glutation;

(d) terpenoids, monoterpenes, diterpenes, sesquiterpenes, triterpenes, tetraterpenes, carotenoids, alfa-carotene, phytoene, cyclo-artenol, betacarotene, ionone, zeaxanthin, capsanthin, astaxanthin, canthaxantin,

(e) violaxanthin, mutatoxanthin, luteoxanthin, auroxanthin, neoxanthin, apocarotinal, xanthophylls;

(f) butylhydroxyanisol, 2,6-di-tert-butylhydroxytoluene, tert-butylhydroquinone, 2,6-di-tert-butylhydroquinone, 2,6-diterbutyl-4-hydroxymethylphenol, 2,4,5-trihidroxibutyro phenone, alpha, beta, gamma and delta tocopherols, alpha, beta, gamma and delta tocotrienols, alpha, beta, gamma and delta tocochromanols;

(g) alpha-lipoic acid, coenzyme Q-10, vitamins, aminoacids, L-arginine, cistine, cisteine, oligopeptides, peptides, carnosine, carnitine, glutathione, enzymes;

enzyme inhibitors, phenolases or oxigenases or lipooxigenases or lipase inhibitors;

(h) minerals and oligoelements, selenium, zinc, magnesium.

10. Microcapsules according to claim 1 characterized in that the microcapsules contain at least one biologically active compound obtained from the following group of organisms:

Medicago sativa, Pimenal officinalis, Hibiscus abelmoschus, Angelica archengelica, Galipea officinalis, Pimpinella anisum, Ferule foetida, Ferula asafetida, Melissa officinalis, Myroxylon pereirae, Ocimum basillicum, Pimenta acris, Citrus aurantium bergamia, Prunus amygdalus, Citrus aurantium, Citrus aurantium amara, Piper nigrum, Prunus spinosa, Anibe rosaeodora, Camelia oleifera, Camelia sinensis, Carum carni, Elettaria cardamomum, Ceratonia siliqua, Daucus carota, Dacus carota sativa, Cascarilla, Apium graveolens, Anthemis nobilis, Matricaria chamomilia, Anthemis nobilis, Anthriscus cerefolium, Cichorium intybus, Cinnamomum spp., Cinnamomum zeylanicum, Cymbopogon nardus, Salvia sciarea, Trifolium pratense, Theobroma cacao, Coffea arabica, Coriandrium sativum, Cumlnum cyminum, Taraxacum officinale, Sambucus nigra, Edelweiss, Helichrysum italicum, Foeniculum vulgare, Trigonella foenumgraecum, Arabidopsis spp., Zingiber officinale, Citrus grandis, Psidium guajava, Humulus lupus, Marrubium vulgare, Monarda punctata, Hyssopus officinals, Jasminum officinale, Jasminum grandiflorum, Juniperus spp., Juniperus comunis, Eucaliptus officinalis, Cola acuminata, Laurus nobilis, Lavandula spp., Lavanduia hybrida, Taxus baccata, Citrus medica limonum, Myristica fragans, Marjorana hortensis, Thymus spp., Thymus officinalis, Thymus mastichina, Ilex paraguarensis, Chamomilla recutita, Saccharum officinarum, Myristica fragans, Allium cepa, Citrus aurantium duicis, Carum petroselinum, Mentha pulegium, Mentha piperita, Pimenta officinalis, Chimaphiia umbellate, Punica granatum, Pelargonium spp., Pelargonium graveolens, Rosmarinus officinalis, Crocus sativus, Salvia spp., Salvia officinalis, Mentha spicata, Mentha viridis, Satureia hortensis, Satureja hortensis, Origanum majorana, Tamarindus indica, Citrus reticulata, Artemisia dracunculus, Thea sinensis, Thymus vulgaris, Polianthes tuberosa, Curcuma longa, Prunus serotina, Thymus serpillum, Satureja montana, Cananga odorata, Curcuma zedoaria, Plantago major, Adansonia digitata, Ananas comosus, Artocarpus altilis, Carica papaya, Lycopersicon esculentum, Cephalophus spp., Vaccinium myrtillus, Thymus aragonensis, Thymus spp., Citrus aurantiifolia, Citrus paradisi, Cucumis melo, Cucurbita spp., Vitis spp., Vitis vinifera, Mangifera indica, Lamiaceae, Coleus ssp., Hedeoma ssp., Hyptis ssp., Leonurus spp., Leucas ssp., Lycopus, ssp., Marrubium spp., Mentha spp., Monarda spp., Periila spp., Prunella spp., Salvia spp., Stachys spp., Teucrium spp., Thymus spp., Cannabis spp., Digitalis lanata, Adonis vernalis, Aesculus hippocastanum, rrazinus rhychophylla, Agrimonia supatoria, Rauvolfia sepentina, Andrographis panicuiata, Areca catechu, Atropa belladonna, Berberis vulgaris, Ardisia japonica, Betula alba, Ananas comosus, Camellia sinensis, Cinnamomum camphora, Camptotheca acuminata, Potentilla fragarioides, Erythroxylum coca, Papaver somniferum, Colchicum autumnale, Claviceps purpurea, Digitalis purpurea, Digitalis lanata, Glaucium flavum, Papaver somniferum, Gossypium spp., Hyoscyamus niger, Camptotheca acuminata, Piper methysticum, Lobelia inflata, Crotalaria sessiliflora, Nicotiana tabacum, Physostigma venenosum, Ephedra sinica, Cinchona ledgeriana, Rhododendron molle, Datura spp., Taxus brevifolia, Strychnos nux-vomica, Stevia rebaudiana, Valeriana officinalis, Pausinystalia yohimbe, Ephedra spp., Crataegus oxyacantha, Hamamelis virginiana, Hydrastis canadensis, Hypericum perforatum, Potentilla erectra, Ledum palustre, Salvia officinalis, Chamomilla recutita, Arctostaphylos uva, Eucommia ulmoides, Mytilus galioprovincialis, Dipiazium esculentum, Manihot utillissima, Sauropous androgynus, Terminalia arjuna, Iberis amara, Crataegus spp., Arbutus unedo, Cynara scolymus, Amaranthus caudatus, Alchornea laxiflora, Alpinia officinarum, Xanthophyilomyces dendrorhous, Crataegus monogyna, Taxus yunnanensis, Bacopa monniera, Cistus albidus, Ocimum basilicum, Thymus vulgaris, Bixa orellana, Centella asiatica, Urtica dioica, Agrocybe aegerita, Crataegus laevigata, Satureja hortensis, Crocus sativus, Coccinia indica, Brugia malayi, Rubus spp., Silybum marianum, Cannabis spp., Cannabis sativa, Hypericum perforatum, Rhus coriaria, Olea europaea, Cyclopia intermedia, Ginkgo biloba, Lentinus lepideus, Pseudomonas putida, sargassum micracanthum, Pinus radiata, Pinus spp., Phaseoulus mungo, Cicer arietinum, Vigna sinensis, Phaseolus aureus, Dolichos lablab, Cajanus cajan, Vicia faba, Dolichos biflorus, Phaseolus lunatus, Phaseolus aconitifolius, Pisum sativum, Psophocarpus tetragonolobus, Arachis hypoagea, Brassica spp., Brassica campestris, Brassica napus, Valeriana officinalis, Echinacea purpurea, Echinacea pallida, Echinacea angustifolia, Glycyrrhiza glabra, Seronea repens, Vaccinium macrocarpon, Tancetum parthenuum, Vaccinium macrocarpon, cereals, seed fruits, silvestre bays, leguminosae, green tea, black tea, microorganisms able to produce long-chained unsaturated fatty acids, Lactobacillus casci, L. acidophillus, L. rhamnosus, L. paracasei, L. gasseri, L. fermentum, L. plantarum, L. salivarius, L. crispatus, L. bulgaricus, L. fermentum, L. reuteri, Bifidobacterium infantis, B. bifidum, Streptococcus termophilus, S. bovis Enterococcus durans, E. faecalis, E. Gallinarum, Esherichia coli, Propionibacterium freudenreicheii, and genetically modified bacteria or fungi or yeasts having inserted genes of probiotic bacteria, Saccharomyces cerevisiae, Kluyveromices marxianus, Rhodotorula rubra, Orobolomvces puniceus, Aureobasidium pullulans, Leucosporidium scotti.

11. Microcapsules according to claim 1 characterized in that they ontain at least one compound selected from the acids:

arachidonic, sterad onic, eicosapentenoic, docosahexenoic, docosapentenoic, linoieic, conjugated linoleic, inolenic, gamma•

linolenic, alpha-linoleic, dihomogamma-linolenic, leic acid;

in any isomeric and/or stereochemical configuration, as well as any ester, ether, glyceride, phospholipid, sphingolipid derivatives thereof.

12. Microcapsules according to claim 1 characterized in that they contain a combination of compounds selected from:

omega-3, omega-6, omega-9 fatty acids and cerebrosides.

13. Microcapsules according to claim 1 characterized in that they contain a mixture of omega-3 and omega-6 fatty acids in a ratio 0.5-10.0, and contain cerebrosides in a percentage of 0.005%-1% with respect to the weight of omega-3 plus omega-6 fatty acids.

14. Microcapsules according to claim 1 characterized in that they release the biologically active material(s) by at least a factor belonging to the group;

pH, temperature, ionic force, osmosis, volatilization or presence of chemicals or enzymes that dissolve the microcapsules'"'"' wall.

15. Microcapsules according to claim 1 characterized in that the inner content of one or more of the first inner microcapsules comprises second inner microcapsules suspended in the second emulsion.

16. Microcapsules according to claim 15 characterized in that the microcapsules are multi-microcapsules having up to 5 degrees of multi-microencapsulation, and wherein each degree of multi-microencapsulation comprises a first microcapsule suspended in an emulsion of water in oil of a second microcapsule.