Device for preparing a sample

• US 8,685,708 B2
• Filed: 04/12/2013
• Issued: 04/01/2014
• Est. Priority Date: 04/18/2012
• Status: Expired due to Fees

• Current Assignee: PathoGenetix, Inc. (Toxic Reports LLC)
• Original Assignee: PathoGenetix, Inc. (Toxic Reports LLC)
• Inventors: Harris, John L., West, Adrian Mark Thomas, Ten Broeck, Dirk Peter, Protozanova, Ekaterina
• Primary Examiner(s): Bhat, Narayan
• Application Number:
  US13/862,081
• Publication Number:
  US 20130288234A1
• Time in Patent Office: 354 Days
• Field of Search: 435/6.1, 435/283.1, 435/288.2, 435/288.5, 435/295.3, 210/650
• US Class Current: 435/283.1
• CPC Class Codes: B01D 11/0415 in combination with membranes
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The invention provides a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA.

Claims

1. A device comprising:

a body having a chamber with an inlet;

a membrane positioned in the body, the membrane having a first side and a second side, wherein the inlet is positioned on the first side of the membrane;

a plurality of channels coupled to the bottom of the chamber, wherein the plurality of channels are positioned on the second side of the membrane, wherein each of the plurality of channels extends outwardly from the membrane, the plurality of channels including at least a first channel and a second channel, wherein the first channel extends outwardly from a central portion of the membrane, and wherein the second channel extends outwardly from a peripheral portion of the membrane,

wherein the membrane includes at least a first zone and a second zone, and there is a barrier which separates the first zone from the second zone, and

wherein the chamber body comprises a plurality of downwardly extending feet spaced around a perimeter of the chamber, with a plurality of passages formed between the feet configured such that fluid can pass from the chamber into the second zone of the membrane.

2. The device of claim 1, wherein the chamber is substantially frustoconical in shape.

3. The device of claim 1, wherein the body further comprises a collar extending upwardly from the chamber inlet, the collar having a passageway extending therethough, wherein the passageway has an inlet configured to receive a sample, wherein the passageway inlet is larger than the chamber inlet.

4. The device of claim 3, wherein at least a portion of the collar passageway is substantially frustoconical in shape as it narrows from the passageway inlet to the chamber inlet.

5. The device of claim 3, wherein the collar is integrally formed with the chamber.

6. The device of claim 1, wherein the membrane includes a substantially annular shaped portion and the barrier is substantially annular in shape.

7. The device of claim 1, wherein the barrier is formed of a weld on the membrane.

8. The device of claim 1, wherein the plurality of channels further comprises a third channel which extends outwardly from the peripheral portion of the membrane.

9. The device of claim 8, wherein the third channel is positioned on the peripheral portion of the membrane opposite the second channel.

10. The device of claim 1, wherein the plurality of channels are formed into a second body which is formed separately from the chamber body.

11. A method comprising:

introducing into the device of claim 1 a nucleic acid comprising sample;

manipulating the sample with one or more reagents; and

eluting nucleic acid from the device.