In vitro techniques for use with stem cells
First Claim
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1. A method for use with extracted blood, comprising:
- applying blood to a first gradient suitable for selecting first-pass white blood cells having a density less than 1.077 g/ml;
applying the entire first-pass white blood cell fraction to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml;
increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 1 and 30 days; and
identifying endothelial progenitor cells in the cultured cells.
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Abstract
A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g/ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.
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Citations
10 Claims
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1. A method for use with extracted blood, comprising:
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applying blood to a first gradient suitable for selecting first-pass white blood cells having a density less than 1.077 g/ml; applying the entire first-pass white blood cell fraction to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 1 and 30 days; and identifying endothelial progenitor cells in the cultured cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification