Multilayered optical sensing patch and retaining plug therefor
First Claim
1. A method of manufacturing a multilayered optical sensing patch, comprising the steps of:
- (a) soaking a porous polymer support membrane in a pH buffer composition, dimethyl formamide, or triethylamine in water solution;
(b) placing the porous polymer support membrane in a solution comprising a reactive indicator, and allowing the porous polymer support membrane to soak in the solution for a time period sufficient to allow the reactive indicator to covalently bond to free amines on the porous polymer support membrane;
(c) removing any unbound reactive indicator from the polymer sensing membrane layer by decanting the solution and/or rinsing the solution from the porous polymer support membrane; and
(d) laminating a heat sealable polymer substrate film to the porous polymer support membrane layer, having pores therein, using a combination of heat and pressure;
said heat sealable polymer substrate film having an optical transparency of at least 50%;
said laminating comprising heating the heat sealable polymer substrate film and the porous polymer support membrane layer to a temperature higher than an onset melting point of the heat sealable polymer substrate film and lower than a melt temperature of the porous polymer support membrane layer.
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Abstract
A multilayered optical sensing patch, for the measurement of conditions, such as pH, oxygen level, etc, within containers, is provided. The multilayered optical sensing patch of the present invention is comprised of a heat sealable polymer substrate layer, and a polymeric sensing membrane later attached thereto. The polymer sensing membrane layer is formed of a porous polymer support membrane, and an optical sensing composition (comprising a reactive indicator) covalently bonded thereto. The heat sealable polymer substrate layer is capable of being securely bonded to the inner layer of bioreactor bags, as well as the porous polymer support substrate layer. Further, the porous polymer support membrane layer provides a firm supporting structure for the polymeric sensing layer, thereby protecting the optical sensing composition disposed therein from degradation/damage.
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Citations
6 Claims
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1. A method of manufacturing a multilayered optical sensing patch, comprising the steps of:
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(a) soaking a porous polymer support membrane in a pH buffer composition, dimethyl formamide, or triethylamine in water solution; (b) placing the porous polymer support membrane in a solution comprising a reactive indicator, and allowing the porous polymer support membrane to soak in the solution for a time period sufficient to allow the reactive indicator to covalently bond to free amines on the porous polymer support membrane; (c) removing any unbound reactive indicator from the polymer sensing membrane layer by decanting the solution and/or rinsing the solution from the porous polymer support membrane; and (d) laminating a heat sealable polymer substrate film to the porous polymer support membrane layer, having pores therein, using a combination of heat and pressure;
said heat sealable polymer substrate film having an optical transparency of at least 50%;
said laminating comprising heating the heat sealable polymer substrate film and the porous polymer support membrane layer to a temperature higher than an onset melting point of the heat sealable polymer substrate film and lower than a melt temperature of the porous polymer support membrane layer.
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2. An optical sensing patch retaining plug comprising:
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a plug body having an exterior plug face surface;
the exterior plug face surface being a surface defining a portion of an outer boundary of the plug body;an optical sensing patch directly disposed on a substantially flat portion of the exterior plug face surface;
optical transparency of material of the portion of the exterior plug face surface on which the optical sensing patch is disposed being selected such that light emitted from a fiber-optic device disposed within a fiber optic insertion channel illuminates the optical sensing patch;
the portion of the exterior plug face surface on which the optical sensing patch is disposed and a portion of the plug face surface peripheral to the portion of the plug face surface on which the optical sensing patch is disposed being comprised of heat sealable material; andthe fiber optic insertion channel being disposed within said plug body, said fiber optic insertion channel being disposed adjacent to the optical sensing patch;
wherein at least a portion of the substantially flat portion of the plug face surface not in communication with the optical sensing patch is configured to be securely attached to a bioreactor bag or other container of interest;
said optical sensing patch being configured to contact contents of the bioreactor bag through an opening in the bioreactor bag or other container of interest. - View Dependent Claims (3, 4, 5, 6)
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Specification