High throughput screening employing combination of dispensing well plate device and array tape

• US 8,685,750 B2
• Filed: 04/28/2009
• Issued: 04/01/2014
• Est. Priority Date: 04/28/2008
• Status: Active Grant

• Current Assignee: Douglas Scientific LLC
• Original Assignee: Douglas Scientific LLC
• Inventors: Schoeneck, Richard Jerome
• Primary Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US12/989,856
• Publication Number:
  US 20110053786A1
• Time in Patent Office: 1,799 Days
• Field of Search: 436/180, 436/47
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

High throughput screening is performed by directing an array tape (60) provided with a plurality of wells arranged in rows and columns into a dispensing well plate device (25) having various row and column actuators (40, 50). With the wells containing samples to be tested, activating select ones of row and column actuators (40, 50) of the dispensing well plate device (25), as well causing relative shifting between the actuators (40, 50) and the array tape (60), enables fluid to be dispensed from one or more nozzles of the dispensing well plate device (25) into predetermined ones of the plurality of wells in a wide range of patterns.

Claims

1. Method comprising:

providing a dispenser containing a plurality of different first reagents arranged in first and second columns and first and second rows defining a first array;

providing first and second targets including wells arranged in first and second columns and first and second rows defining a second array and including second reagents, with the first and second columns and the first and second rows of the first array corresponding to the first and second columns and the first and second rows of the second array;

aligning the dispenser with the first array of the first reagents with the wells of the first target and actuating the dispenser to dispense the first reagents into the wells of the first target;

moving the first and second targets relative to the dispenser in a direction parallel to the first and second columns of the first and second targets; and

aligning the dispenser with the first column of the first array of first reagents with the second column of the wells of the second target and actuating the dispenser to dispense the first column of first reagents into the wells of the second column of the second target.

2. The method of claim 1 further comprising:

aligning the dispenser with the second column of the first array of first reagents with the first column of the wells of the second target and actuating the dispenser to dispense the second column of first reagents into the wells of the first column of the second target.

3. The method of claim 2 wherein providing the first target comprises providing a plurality of different second reagents arranged in the first and second columns and the first and second rows.

4. The method of claim 3 further comprising:

aligning the dispenser with the first row aligned with the second row of the wells of the second target and actuating the dispenser to dispense the first row into the second row of the wells of the second target; and

aligning the dispenser with the second row aligned with the first row of the wells of the second target and actuating the dispenser to dispense the second row into the first row of the wells of the second target.

5. The method of claim 1 wherein providing the first and second targets comprises providing a carrier tape including the first and second targets.

6. The method of claim 1 wherein providing the dispenser comprises providing a dispensing well plate containing the plurality of different first reagents and including actuators;

and wherein actuating the dispenser comprises actuating the actuators to dispense the first reagents from the dispensing well plate.

7. The method of claim 1 wherein providing the dispenser comprises:

providing a dispensing well plate including a plurality of nozzles arranged in the first and second rows and the first and second columns of the first array;

providing the first reagents in a prep vial;

placing the prep vial relative to the dispensing well plate with the plurality of nozzles located in the first reagent; and

drawing the first reagents from the prep vial to at least one of the plurality of nozzles of the dispensing well plate.

8. The method of claim 7 wherein drawing the first reagents comprises drawing the first reagents from the prep vial to the nozzles of the first column of the first array.

9. Method comprising:

providing a dispenser with a plurality of different first reagents arranged with a first spacing between the different first reagents and in a column;

providing a dispenser with a plurality of different second reagents arranged with a second spacing between the different second reagents and in a row;

providing first and second targets each including wells in an array having first and second columns and first and second rows, with the wells in the first and second columns being spaced the first spacing of the first reagents and the wells in the first and second rows being spaced corresponding to the second spacing of the second reagents;

aligning the dispenser with the column of the plurality of different first reagents with the first column in the first target and actuating the dispenser to dispense the first reagents into the first column in the first target;

aligning the dispenser with the column of the plurality of different first reagents with the second column in the second target and actuating the dispenser to dispense the first reagents into the second column in the second target;

aligning the dispenser with the row of the plurality of different second reagents with the first row in the first target and actuating the dispenser to dispense the second reagents into the first row in the first target; and

aligning the dispenser with the row of the plurality of different second reagents with the second row in the second target and actuating the dispenser to dispense the second reagents into the second row in the second target.

10. The method of claim 9 wherein providing the dispenser with the plurality of different first reagents comprises providing the dispenser with the plurality of different first reagents arranged in first and second columns.

11. The method of claim 9 wherein providing the dispenser with the plurality of different second reagents comprises providing the dispenser with the plurality of different second reagents arranged in first and second rows.

12. The method of claim 9 wherein providing the first and second targets comprises providing a carrier tape including the first target and the second target.

13. The method of claim 9 wherein providing the dispenser with the plurality of different first reagents comprises:

providing a dispensing well plate including a plurality of nozzles arranged in the column of the dispenser;

providing the first reagent in a prep vial;

placing the prep vial relative to the dispensing well plate; and

drawing the first reagents from the prep vial to the plurality of nozzles arranged in the column of the dispenser.