Coupling agents for orthopedic biomaterials

• US 8,686,064 B2
• Filed: 09/18/2007
• Issued: 04/01/2014
• Est. Priority Date: 10/08/2002
• Status: Active Grant

• Current Assignee: Warsaw Orthopedic Incorporated (Medtronic Plc)
• Original Assignee: Warsaw Orthopedic Incorporated (Medtronic Plc)
• Inventors: Shimp, Lawrence A., Knaack, David
• Primary Examiner(s): PEPITONE, MICHAEL F
• Application Number:
  US11/856,977
• Publication Number:
  US 20080009955A1
• Time in Patent Office: 2,387 Days
• Field of Search: 523/116, 523/113, 523/115, 623/23.61, 623/23.63, 424/423, 424/549
• US Class Current: 523/116
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 27/3608 Bone, e.g. demineralised bone matrix [DBM], bone powder
• CPC Class Codes: A61L 27/365 Bones
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3687 characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
• CPC Class Codes: A61L 27/3821 Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/44 having a macromolecular matrix
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers

Abstract

The invention provides a method for the preparation of bone-polymer composites wherein the mineral portion of the bone is treated with a coupling agent before being incorporated into a biocompatible polymeric matrix. The resulting composites may be used as such or be further processed to form an osteoimplant.

Claims

1. A method for producing a bone-polymer composite comprising:

(a) providing a plurality of bone particles;

(b) treating the bone particles with a silane coupling agent such that the silane coupling agent is directly coupled to the bone particles; and

(c) incorporating the bone particles obtained from (b) into a polymer, wherein the polymer is not a polyaromatic polymer.

2. The method of claim 1, wherein the bone particles are obtained from one or more of autologous bone, allogenic bone, xenogenic bone, and mixtures thereof.

3. The method of claim 1, wherein the bone particles are obtained from one or more of cortical bone, cancellous bone, cortico-cancellous bone, and mixtures thereof.

4. The method of claim 1, wherein the bone particles are obtained from one or more of nondemineralized bone, deorganified bone, anorganic bone, and mixtures thereof.

5. The method of claim 1, wherein the coupling agent is a silane selected from the group consisting of silanes bearing one hydrolyzable or leaving group, silanes bearing two hydrolyzable or leaving groups, and silanes bearing three hydrolyzable or leaving groups.

6. The method of claim 1, wherein the polymer is a biocompatible polymer selected from the group consisting of polymers of natural origin, polymers of artificial origin, and any combination of natural and artificial polymers.

7. The method of claim 1, wherein the polymer is selected from the group consisting of biodegradable polymers, non-biodegradable polymers, co-polymers of biodegradable polymers, co-polymers of non-biodegradable polymers, and co-polymers of biodegradable and non-biodegradable polymers.

8. The method of claim 1, wherein the polymer is a natural polymer selected from the group consisting of polysaccharides and proteins.

9. The method of claim 8, wherein the polymer is selected from the group consisting of starch, dextran, cellulose, gelatin, and collagen.

10. The method of claim 1, wherein the polymer is an artificial polymer selected from the group consisting of poly(anhydrides), poly(hydroxy acids), polyesters, poly(orthoesters), polycarbonates, poly(propylene fumerates), poly(caprolactones), polyamides, polyamino acids, polyacetals, polylactides, polyglycolides, polysulfones, poly(dioxanones), polyhydroxybutyrates, polyhydroxyvalyrates, poly(vinyl pyrrolidones), biodegradable polycyanoacrylates, biodegradable polyurethanes, polysaccharides, tyrosine-based polymers, poly(methyl vinyl ether), poly(maleic anhydride), poly(glyconates), polyphosphazines, poly(esteramides), polyketals, poly(orthocarbonates), poly(maleic acid), poly(alkylene oxalates), poly(alkylene succinates), nonbiodegradable polyurethanes, polyureas, poly(ethylene vinyl acetate), polypropylene, polymethacrylate, polyethylene, poly(ethylene oxide), and co-polymers, adducts, and mixtures thereof.

11. The method of claim 5, wherein the silane coupling agent attached to the bone particles is linked to the polymer by a member of covalent and non-covalent interactions.

12. The method of claim 1, further comprising the step of modifying a surface of at least a portion of the bone particles before treatment with the coupling agent.

13. The method of claim 12, wherein modifying comprises treating the bone particles with dilute phosphoric acid.

14. The method of claim 1, further comprising the step of modifying a composition of the bone particles before treatment with the coupling agent.

15. The method of claim 14, wherein modifying comprises one or more of drying the bone particles, lyophilizing the bone particles, defatting the bone particles, treating the bone particles with a detergent, treating the bone particles with a solvent, treating the bone particles with a surfactant, removing pathogens, and inactivating pathogens.

16. The method of claim 15, wherein removing pathogens comprises one or more of radiation sterilization, antibiotic treatment, and treatment with a pathogen-inactivating chemical.

17. The method of claim 15, wherein inactivating pathogens comprises one or more of radiation sterilization, antibiotic treatment, and treatment with a pathogen inactivating chemical.

18. The method of claim 1, wherein step (c) further includes combining a cross-linking agent with treated bone particles and the polymer.

19. The method of claim 18, wherein the cross-linking agent is selected from the group consisting of aldehydes, polyepoxy compounds, polyvalent metallic oxides, organic tannins, N-hydroxysuccinimides, N-hydroxysulfosuccinimides, phenolic oxides, hydrazides, carbodiimides, isocyanates, isothiocyanates, sugars, and enzymes.

20. The method of claim 1, wherein step (c) comprises solvent casting, melting, or both.

21. The method of claim 1, further comprising combining the composite with one or more of a wetting agent, biocompatible binder, filler, fiber, plasticizer, biostatic/biocidal agent, surface active agent, biomolecule, small molecule, and bioactive agent.

22. The method of claim 21, wherein the biologically active agent is selected from the group consisting of antibiotics, chemotherapeutics, bone cell inducers, and bone cell stimulators.

23. The method of claim 1, further comprising incorporating osteoblasts into the composite.

24. The method of claim 1, further comprising processing the composite to obtain an osteoimplant having a desired shape.

25. The method of claim 1, wherein the silane coupling agent has a formula:

R_(4-m)SiR′

_m

wherein R is a non hydrolyzable organic functional group;

R′

is a hydrolyzable or good leaving functional group; and

m is an integer from 1 to 3.

26. A method for producing a bone-polymer composite comprising:

(a) providing a plurality of bone particles;

(b) treating the bone particles with a silane coupling agent such that the silane coupling agent is directly coupled to the bone particles; and

(c) incorporating the bone particles obtained from (b) into a polymer;

wherein the polymer is not a polyaromatic polymer, and wherein the polymer is an artificial polymer selected from the group consisting of poly(caprolactones), polylactides, polyglycolides, biodegradable polyurethanes, nonbiodegradable polyurethanes, and co-polymers, adducts, and mixtures thereof.

27. A method for producing a bone-polymer composite comprising:

(a) providing a plurality of bone particles;

(b) treating the bone particles with a silane coupling agent such that the silane coupling agent is directly coupled to the bone particles; and

(c) incorporating the bone particles obtained from (b) into a polymer, wherein the polymer is not a polyaromatic polymer, and wherein the bone particles represent about 60% to about 75% of the total weight of the composite.