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Methods for the separation of biological molecules using sulfolane

  • US 8,686,129 B2
  • Filed: 03/20/2007
  • Issued: 04/01/2014
  • Est. Priority Date: 03/20/2007
  • Status: Active Grant
First Claim
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1. A method for the separation of a nucleic acid from other biological molecules present in a sample, said method comprising:

  • applying an aqueous sample comprising at least one nucleic acid and at least one other biological molecule to at least one mineral substrate in the presence of one or more salts and sulfolane, wherein the concentration of the sulfolane is 15% to 80% by volume, and wherein the mineral substrate adsorbs at least one nucleic acid to create a mineral substrate-nucleic acid complex and a composition not adsorbed to the mineral substrate; and

    removing at least a portion of the composition not adsorbed to the mineral substrate, thereby separating the nucleic acid from at least one other biological molecule present in the sample.

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