Corn plant MON88017 and compositions and methods for detection thereof

• US 8,686,230 B2
• Filed: 06/21/2012
• Issued: 04/01/2014
• Est. Priority Date: 12/15/2003
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Beazley, Kim A., Coombe, Timothy R., Groth, Mark E., Hinchey, Terri B., Pershing, Jay C., Vaughn, Ty T., Zhang, Bei
• Primary Examiner(s): Kruse, David H
• Application Number:
  US13/529,933
• Publication Number:
  US 20130031679A1
• Time in Patent Office: 649 Days
• Field of Search: None
• US Class Current: 800/300.1
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

Claims

1. A recombinant DNA molecule comprising a nucleic acid sequence encoding a CRY3Bb1 protein and a CP4 EPSPS protein, said recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4 and SEQ ID NO;

5, or a full complement thereof.

3. The recombinant DNA molecule of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

1.

4. The recombinant DNA molecule of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

2.

5. The recombinant DNA molecule of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

3.

6. The recombinant DNA molecule of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

4.

7. The recombinant DNA molecule of claim 1, comprising the nucleic acid sequence of SEQ ID NO:

5.

2. A composition comprising a pair of DNA primer molecules comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first DNA molecule comprises a nucleotide sequence of sufficient length of contiguous nucleotides of a transgene insert DNA sequence of SEQ ID NO:

5, or a full complement thereof and said second DNA molecule comprises a nucleotide sequence of sufficient length of contiguous nucleotides of the maize genomic DNA sequence of SEQ ID NO;

3 or SEQ ID NO;

4, to function as DNA primers when used together in a DNA amplification method to produce an amplicon diagnostic for corn event MON88017 DNA, wherein the amplicon comprises SEQ ID NO;

1 or SEQ ID NO;

2.

8. A transgenic corn plant, seed, or part thereof comprising a DNA molecule comprising the nucleotide sequence of SEQ ID NO:

5.

9. The transgenic corn plant, seed, or part thereof of claim 8, wherein the part comprises pollen, ovule, seed, roots, or leaves.

10. A commodity or foodstuff produced from the transgenic corn plant, seed, or part thereof of claim 8 and comprising a DNA molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4 and SEQ ID NO;

5, or a full complement thereof.

11. The commodity or foodstuff of claim 10, selected from the group consisting of corn oil, corn starch, corn meal, corn flour, a cosmetic, and a bulking agent.