Apparatus and method for determining the results of assays

• US 8,689,614 B2
• Filed: 08/26/2008
• Issued: 04/08/2014
• Est. Priority Date: 08/24/2007
• Status: Active Grant

• Current Assignee: Highland Biosciences Limited
• Original Assignee: Highland Biosciences Limited
• Inventors: Day, Richard, Day, Emma
• Primary Examiner(s): Williams, Hezron E
• Assistant Examiner(s): NGUYEN, HOANG T
• Application Number:
  US12/675,051
• Publication Number:
  US 20110129929A1
• Time in Patent Office: 2,051 Days
• Field of Search: 73/540.4, 73/541.1, 73 5424- 27, 435/13, 435/283.1, 436 8- 19
• US Class Current: 73/54.01
• CPC Class Codes: G01N 11/16 by measuring damping effect upon oscillatory body
• CPC Class Codes: G01N 2291/02818 Density, viscosity
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 9/002 using variation of the resonant frequency of an element vibrating in contact with the material submitted to analysis G01N9/34 takes precedence
• CPC Class Codes: G01N 9/36 Analysing materials by measuring the density or specific gravity, e.g. determining quantity of moisture methods of measurement in general G01N9/02 - G01N9/32
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

The present invention provides a method for the real-time continuous monitoring of a change or density and/or viscosity within a test sample. Such methods can be used to determine the occurrence of a chemical reaction within a test sample where the same causes and increase or decrease in the density and/or viscosity of the sample due to, for example, a gelation, precipitation or coagulation occurring within the test sample. There is further provided a multi-resonator apparatus for use in measuring the density and/or viscosity of a test sample in which the multi-beam resonator is immersed.

Claims

1. An apparatus for use in monitoring a change in a density and/or a viscosity of a test sample, comprising:

a multi-beam resonator, the multi-beam resonator comprising;

a base substrate comprising a mounting zone defined thereon having a defined longitudinal length;

at least two resonant beam members, wherein each of the resonant beam members has a respective longitudinal length, wherein a ratio of the longitudinal length of each resonant beam member to the defined longitudinal length of the mounting zone of the base substrate is in a range of 1 to 9;

at least one vibratory element positioned to cause a vibration of at least one of the resonant beam members;

at least one sensor means for monitoring at least one parameter associated with the vibration of the resonant beam member; and

a reaction chamber for receiving and retaining a test sample, the reaction chamber comprising at least one inlet port to allow an introduction of the test sample; and

a reagent comprising amebocyte lysate or a synthetic analogue thereof for adding to the test sample to form a test sample mixture within the reaction chamber.

2. An apparatus as claimed in claim 1, wherein the resonant beam members are provided in a parallel arrangement and are fixed at each end of their respective longitudinal lengths to the base substrate.

3. An apparatus as claimed in claim 1, wherein the at least one inlet port allows the test sample to be introduced into the reaction chamber from a top of the reaction chamber.

4. An apparatus as claimed in claim 1, wherein at least one outlet port is provided in the reaction chamber, wherein the at least one outlet port is provided in an arrangement and of dimensions which are suitable to permit air to escape from the reaction chamber.

5. An apparatus as claimed in claim 1 wherein the ratio of the total longitudinal length of the resonant beam members to the length of the mounting zone of the multi-beam resonator which is defined on the base substrate is in the range of 1 to 9.

6. An apparatus as claimed in claim 1, wherein the ratio of the longitudinal length of each of the resonant beam members to the defined longitudinal length of the mounting zone of the multi-beam resonator is about 4.

7. An apparatus as claimed in claim 1, wherein a distance of a spacing between an outer lateral side of an outermost resonant beam member and an innermost wall of a surrounding housing is 5 mm or less.

8. An apparatus as claimed in claim 1, wherein a distance of a spacing between an outer lateral side of an outermost resonant beam member and an innermost wall of a surrounding housing is 0.5 mm or less.

9. An apparatus as claimed in claim 1, wherein a distance of a spacing between an outer lateral side of an outermost resonant beam member and an innermost wall of a surrounding housing is greater than 25 μ

m.

10. An apparatus as claimed in claim 1, wherein an internal height of the reaction chamber is greater than 1 mm.

11. An apparatus as claimed in claim 1, wherein the multi-beam resonator comprises three resonant beam members, wherein one of the three resonant beam members is a central resonant beam member located between the other two resonant beam members, wherein a width of the central resonant beam member in a lateral direction is about 2 mm or less.

12. An apparatus as claimed in claim 1, wherein the multi-beam resonator comprises three resonant beam members, wherein one of the three resonant beam members is a central resonant beam member located between the other two resonant beam members, wherein a width of the central resonant beam member in a lateral direction is 1 mm.

13. An apparatus as claimed in claim 1, wherein the multi-beam resonator comprises three resonant beam members, wherein one of the three resonant beam members is a central resonant beam member located between the other two resonant beam members, wherein two of the three resonant beam members are outer resonant beam members located on an outside of the central resonant beam member, wherein each resonant beam member has a lateral width, and wherein the lateral width of the central resonant beam member is a sum of the lateral widths of the outer resonant beam members.

14. An apparatus as claimed in claim 1, wherein the longitudinal length of at least one of the resonant beam members is 18 mm or less.

15. An apparatus as claimed in claim 1, wherein the longitudinal length of at least one of the resonant beam members is about 5.5 mm or less.

16. An apparatus as claimed in claim 1, wherein the resonant beam members are provided in a parallel arrangement, and wherein a distance of a spacing between resonant beam members is 2 mm or less.

17. An apparatus as claimed in claim 1, wherein a total longitudinal length of the multi-beam resonator is 20 mm or less.

18. An apparatus as claimed in claim 1, wherein a surface of at least part of the reaction chamber is provided with a coating selected from the group of:

a hydrophobic coating and a hydrophilic coating.

20. An apparatus as claimed in claim 1, wherein the amebocyte lysate or the synthetic analogue thereof comprises Limulus amebocyte lysate (LAL) or a synthetic analogue thereof or Tachypleus amebocyte lysate (TAL) or a synthetic analogue thereof.

21. An apparatus as claimed in claim 1, wherein the reagent is provided in a dried form.

22. An apparatus as claimed in claim 1, wherein at least one surface of the reaction chamber is at least partially coated with the reagent.

23. An apparatus as claimed in claim 1, wherein the reagent is a dried amebocyte lysate or synthetic analogue thereof which is provided within the reaction chamber and is reconstituted following addition of a liquid test sample.

24. An apparatus as claimed in claim 1, wherein the amebocyte lysate or synthetic analogue thereof is provided in a liquid solution.

25. An apparatus as claimed in claim 1, wherein the amebocyte lysate or synthetic analogue thereof is added to the reaction chamber prior to, simultaneously, or following loading of the reaction chamber with test sample.

26. An apparatus as claimed in claim 1, comprising the test sample mixture, the test sample mixture being formed by mixing the test sample with the amebocyte lysate or the synthetic analogue thereof prior to loading the test sample mixture into the reaction chamber.

27. An apparatus as claimed in claim 1, wherein the multi-beam resonator comprises at least three resonant beam members.

28. An apparatus as claimed in claim 1, wherein the reaction chamber defines a static volume and allows a defined amount of test sample to be retained within the reaction chamber.

29. An apparatus as claimed in claim 28, wherein an internal volume of the reaction chamber is less than 1000 μ

l.

30. An apparatus as claimed in claim 1, wherein the at least one sensor means is configured to monitor the at least one parameter within a time period of 60 seconds or less.

31. An apparatus as claimed in claim 2, wherein the resonant beam members are an integral part of the base substrate.

32. An apparatus as claimed in claim 1, wherein the sensor means comprises a sensor means for monitoring at least one parameter selected from the group of resonant frequency, quality factor, resonant phase angle, change in resonant frequency, change in quality factor, change in resonant phase angle, rate of change of resonant frequency, rate of change of quality factor, and rate of change in resonant phase angle.

33. An apparatus as claimed in claim 1, wherein the at least one parameter is used to generate a signal to indicate a presence or an absence of bacterial endotoxin in the test sample.

34. An apparatus as claimed in claim 1, wherein the resonant beam members are substantially composed of inert material.

35. An apparatus as claimed in claim 34, wherein the inert material is selected from the group of silicon, copper, palladium, platinum, steel, and stainless steel.

36. An apparatus as claimed in claim 1, wherein the at least one vibratory element is a piezoelectric element.

37. An apparatus as claimed in claim 36, wherein the piezoelectric element comprises piezoelectric material selected from the group of a polymer, PVDF, a crystal, a ceramic, PZT, and a screen printed PZT.

19. A test strip comprising:

a multi-beam resonator, the multi-beam resonator comprising;

a base substrate comprising a mounting zone defined thereon having a defined longitudinal length;

at least two resonant beam members, wherein each of the resonant beam members has a respective longitudinal length, wherein a ratio of the respective longitudinal length of each resonant beam member to the defined longitudinal length of the mounting zone of the base substrate is in a range of 1 to 9;

at least one vibratory element positioned to cause a vibration of at least one of the beam members;

at least one sensor means for monitoring at least one parameter associated with the vibration of the resonant beam member; and

a reaction chamber for receiving and retaining a test sample, the reaction chamber comprising at least one inlet port to allow an introduction of the test sample; and

a reagent comprising amebocyte lysate or a synthetic analogue thereof for adding to the test sample to form a test sample mixture within the reaction chamber.

38. An apparatus for monitoring a change in a density and/or a viscosity of a test sample comprising a multi-beam resonator,

the multi-beam resonator comprising:

a base substrate comprising a mounting zone defined thereon having a defined longitudinal length;

at least two resonant beam members, wherein each of the resonant beam members has a respective longitudinal length, wherein a ratio of the longitudinal length of each resonant beam member to the defined longitudinal length of the mounting zone of the base substrate is in a range of 1 to 9;

at least one vibratory element positioned to cause a vibration of at least one of the resonant beam members,

at least one sensor means for monitoring at least one parameter associated with the vibration of the resonant beam member,

a reaction chamber for receiving and retaining a test sample, the reaction chamber comprising at least one inlet port to allow an introduction of the test sample.

39. An apparatus as claimed in claim 38, wherein a ratio of the longitudinal length of each of the resonant beam members to the defined longitudinal length of the mounting zone of the multi-beam resonator is about 4.

40. An apparatus as claimed in claim 38 wherein a distance of a spacing between an outer lateral side of an outermost resonant beam member and an innermost wall of a surrounding housing is 5 mm or less.

41. An apparatus as claimed in claim 38 wherein an internal height of the reaction chamber is greater than 1 mm.

42. An apparatus as claimed in claim 38 wherein the multi-beam resonator comprises three resonant beam members, wherein one of the three resonant beam members is a central resonant beam member located between the other two resonant beam members, wherein a width of the central resonant beam member in the lateral direction is about 2 mm or less.

43. An apparatus as claimed in claim 38 wherein the multi-beam resonator comprises three resonant beam members, wherein one of the three resonant beam members is a central resonant beam member located between the other two resonant beam members, wherein two of the three resonant beam members are outer resonant beam members located on an outside of the central resonant beam member, wherein each resonant beam member has a lateral width, and wherein the lateral width of the central beam member is a sum of the lateral widths of the outer beam members.

44. An apparatus as claimed in claim 38 wherein the longitudinal length of at least one of the resonant beam members is 18 mm or less.

45. An apparatus as claimed in claim 38 wherein the resonant beam members are provided in a parallel arrangement, and wherein a distance of a spacing between resonant beam members is 2 mm or less.

46. An apparatus as claimed in claim 38 wherein a total longitudinal length of the multi-beam resonator is 20 mm or less.