Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit
First Claim
1. A method for electrochemically measuring a concentration of an analyte in a blood sample using a sensor chip, the method comprising:
- electrochemically detecting a current A reflecting a hematocrit value of the blood sample, in a state where a reductant of a redox substance is present on a working electrode and substantially not in contact with a counter electrode, and of an oxidant of a redox substance is present on the counter electrode and substantially not in contact with the working electrode, so as to obtain data A representing the current A or an equivalent of the current A and corresponding to the hematocrit value;
electrochemically detecting a current B that results from the oxidation or reduction of the analyte in the blood sample caused, in the presence of an oxidant of a redox substance on both of a working electrode and a counter electrode, by a redox enzyme that uses the analyte as a substrate, so as to obtain data B representing the current B or an equivalent of the current B; and
determining a concentration of the analyte in the blood sample based on data C obtained by correcting the data B using the data A.
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Accused Products
Abstract
Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) having branch parts (18a, 18b). A first reagent (13) consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (18b), and a second reagent (19) consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part (18a).
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Citations
4 Claims
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1. A method for electrochemically measuring a concentration of an analyte in a blood sample using a sensor chip, the method comprising:
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electrochemically detecting a current A reflecting a hematocrit value of the blood sample, in a state where a reductant of a redox substance is present on a working electrode and substantially not in contact with a counter electrode, and of an oxidant of a redox substance is present on the counter electrode and substantially not in contact with the working electrode, so as to obtain data A representing the current A or an equivalent of the current A and corresponding to the hematocrit value; electrochemically detecting a current B that results from the oxidation or reduction of the analyte in the blood sample caused, in the presence of an oxidant of a redox substance on both of a working electrode and a counter electrode, by a redox enzyme that uses the analyte as a substrate, so as to obtain data B representing the current B or an equivalent of the current B; and determining a concentration of the analyte in the blood sample based on data C obtained by correcting the data B using the data A. - View Dependent Claims (2, 3, 4)
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Specification