T-cell vaccination with viral vectors via mechanical epidermal disruption
First Claim
1. A method for inducing or stimulating a T cell mediated immune response to an exogenous T cell antigen in human epithelial tissues selected from the group consisting of skin, lung, oral mucosa, gastrointestinal tract, and reproductive mucosa comprising administering to mechanically disrupted human epithelial tissue a live, modified poxvirus expressing the antigen to cause a local infection in the epithelial tissue in an amount sufficient to cause the infected cells to express viral proteins, the exogenous T cell antigen and inflammatory factors and stimulate a T cell immune response in the lymph nodes to the exogenous T cell antigen, wherein all of the virus is 100% replication deficient in normal primary human cells.
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Accused Products
Abstract
Attenuated, replication-deficient viruses such as vaccinia viruses are used to deliver an exogenous viral, bacterial, parasitic or tumor antigen to an epidermal tissue such as the skin, lungs or gastrointestinal tract, which has been mechanically disrupted, in an amount effective to elicit or stimulate a cell mediated immune response. The epidermal tissue may be mechanically disrupted by a device such as a scarification needle or an abrader device. The epidermis may be mechanically disrupted prior to, at the same time, or immediately after the administration of the vaccine. The vaccine can be used to induce immunity against a pathogen, such as a virus, bacteria, or parasite, or against a cancer in a subject that has or is at risk of developing cancer. In some embodiments a co-stimulatory molecule, a growth factor, an adjuvant and/or a cytokine is administered before, with or after the viral vaccine. In some embodiments, the co-stimulatory molecule is co-expressed with the antigen by the virus.
33 Citations
14 Claims
- 1. A method for inducing or stimulating a T cell mediated immune response to an exogenous T cell antigen in human epithelial tissues selected from the group consisting of skin, lung, oral mucosa, gastrointestinal tract, and reproductive mucosa comprising administering to mechanically disrupted human epithelial tissue a live, modified poxvirus expressing the antigen to cause a local infection in the epithelial tissue in an amount sufficient to cause the infected cells to express viral proteins, the exogenous T cell antigen and inflammatory factors and stimulate a T cell immune response in the lymph nodes to the exogenous T cell antigen, wherein all of the virus is 100% replication deficient in normal primary human cells.
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14. A kit for immunization by mechanical disruption of an epidermal tissue consisting essentially of
a device comprising scarification needles or an abrader, or a device comprising microneedles or microprotrusions, wherein the device is suitable for mechanically disrupting a human subject'"'"'s epidermal tissue and a dosage unit of a live, modified, poxvirus expressing an antigen in an effective amount for inducing or stimulating a T cell mediated immune response to an exogenous T cell antigen in human epithelial tissues selected from the group consisting of skin, lung, oral mucosa, gastrointestinal tract, and reproductive mucosa, wherein all of the virus is 100% replication deficient in normal primary human cells.
Specification