Production of carotenoids in oleaginous yeast and fungi
First Claim
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1. An engineered Y. lipolytica strain that produces at least one retinolic compound selected from the group consisting of retinol, retinal, retinoic acid, retinyl palmitate and combinations thereof, the strain comprising at least one retinologenic modification selected from the group consisting of increased expression or activity of a beta-carotene 15,15′
- -monooxygenase polypeptide, increased expression or activity of a retinol dehydrogenase polypeptide, and combinations thereof;
wherein the engineered strain can accumulate lipid to at least about 20% of its dry cell weight; and
wherein as a result of genetic engineering, the engineered strain produces the at least one retinolic compound to a level at least about 1% of its dry cell weight.
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Abstract
The present invention provides systems for producing engineered oleaginous yeast or fungi that express carotenoids.
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Citations
20 Claims
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1. An engineered Y. lipolytica strain that produces at least one retinolic compound selected from the group consisting of retinol, retinal, retinoic acid, retinyl palmitate and combinations thereof, the strain comprising at least one retinologenic modification selected from the group consisting of increased expression or activity of a beta-carotene 15,15′
- -monooxygenase polypeptide, increased expression or activity of a retinol dehydrogenase polypeptide, and combinations thereof;
wherein the engineered strain can accumulate lipid to at least about 20% of its dry cell weight; and wherein as a result of genetic engineering, the engineered strain produces the at least one retinolic compound to a level at least about 1% of its dry cell weight. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- -monooxygenase polypeptide, increased expression or activity of a retinol dehydrogenase polypeptide, and combinations thereof;
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19. An engineered Y. lipolytica strain that produces at least one retinolic compound selected from the group consisting of retinol, retinal, retinoic acid, retinyl palmitate and combinations thereof, the strain comprising at least one genetic modification selected from the group consisting of:
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increased expression or activity of a beta-carotene 15,15′
-monooxygenase polypeptide;increased expression or activity of a retinol dehydrogenase polypeptide; increased expression or activity of phytoene synthase; increased expression or activity of lycopene cyclase; increased expression or activity of phytoene dehydrogenease; increased expression or activity of geranylgeranylpyrophosphate synthase; increased expression or activity of truncated HMG-CoA reductase; and combinations thereof; wherein the engineered strain can accumulate lipid to at least about 20% of its dry cell weight; and wherein as a result of genetic engineering, the engineered strain produces the at least one retinolic compound to a level at least about 1% of its dry cell weight. - View Dependent Claims (20)
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Specification