Microparticles with enhanced covalent binding capacity and their uses
First Claim
1. A product comprising:
- a. A microparticle; and
b. Bovine Serum Albumin (BSA) covalently attached to said microparticle, wherein said BSA comprises activated binding sites capable of forming a covalent bond with a primary amino group.
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Accused Products
Abstract
A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.
548 Citations
3 Claims
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1. A product comprising:
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a. A microparticle; and b. Bovine Serum Albumin (BSA) covalently attached to said microparticle, wherein said BSA comprises activated binding sites capable of forming a covalent bond with a primary amino group. - View Dependent Claims (2, 3)
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Specification
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- Resources
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Current AssigneeBioArray Solutions Limited (Werfen SA)
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Original AssigneeBioArray Solutions Limited (Werfen SA)
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InventorsWang, Xinwen, Banerjee, Sukanta
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Primary Examiner(s)DESAI, ANAND U
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Application NumberUS12/795,198Publication NumberTime in Patent Office1,401 DaysField of SearchNoneUS Class Current514/2CPC Class CodesC07H 21/04 with deoxyribosyl as saccha...C12Q 1/6834 Enzymatic or biochemical co...C12Q 2563/119 the label being proteinicG01N 33/54353 with ligand attached to the...
