Implant of cross-linked spider silk threads

• US 8,696,762 B2
• Filed: 12/11/2006
• Issued: 04/15/2014
• Est. Priority Date: 12/11/2006
• Status: Expired due to Fees

• Current Assignee: Medizinische Hochschule Hannover
• Original Assignee: Medizinische Hochschule Hannover
• Inventors: Vogt, Peter, Allmeling, Christina, Reimers, Kerstin
• Primary Examiner(s): ANDERSON, GREGORY A
• Application Number:
  US12/518,638
• Publication Number:
  US 20100228359A1
• Time in Patent Office: 2,682 Days
• Field of Search: 623/23.72
• US Class Current: 623/23.72
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 2430/16 for reconstruction of eye parts, e.g. intraocular lens, cornea
• CPC Class Codes: A61L 26/0047 Specific proteins or polypeptides not covered by groups A61L26/0033 - A61L26/0042
• CPC Class Codes: A61L 26/0057 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin

Abstract

The present invention provides the use of spider silk threads for the production of tissue implants for medical use. A scaffolding matrix is provided for implantation, which matrix is comprised of spider silk thread and/or segments of spider silk threads which are cross-linked. The cross-linked spider silk thread segments essentially maintain a three-dimensional shape into which it is formed by pressing between molds. The three-dimensional shape obtainable by molding sufficiently stable to be used as an implant or for colonizing by mammalian cells while essentially maintaining the three-dimensional shape. In a further embodiment, the scaffolding matrix of cross-linked spider silk segments is at least partially covered by cells autologous or heterologous to the implant recipient. The matrix colonized with cells is obtainable by in vitro cultivation of isolated cells in suspension with the scaffolding matrix.

Claims

1. Method for the production of implants for medical use, comprising:

mixing spider silk thread segments and a cross-linking agent in the absence of any bulk matrix compound to provide a scaffolding matrix, and molding the scaffolding matrix of cross-linked spider silk threads into a pre-determined three-dimensional form, with no addition of a bulk matrix compound.

2. Method according to claim 1, wherein the scaffolding matrix comprises adherent mammalian cells by in vitro cultivation.

3. Method according to claim 2, wherein the cells are obtained from the future recipient of the implant or are immunologically tolerated heterologous cells.

4. Method according to claim 2 wherein the mammalian cells are obtained from the future recipient and the cultivation comprises incubating the scaffolding matrix with the cells under cell culture conditions.

5. Method according to claim 1, wherein the spider silk thread segments have a length in the range of 0.1 mm to 200 mm.

6. Method according to claim 1, wherein said molding comprises using a die cavity formed between two half moulds.

7. Method according to claim 1 for the replacement of a tissue defect.

8. Implant for medical use comprising spider silk threads, characterized in that the implant is a scaffolding matrix having a predetermined three-dimensional form, obtainable by moulding spider silk threads obtainable from spiders in the presence of a cross-linking agent, with no addition of a bulk matrix compound.

9. The implant of claim 8, applied to a seroserous or serosanguineous wound.

10. The implant of claim 8, wherein the spider silk threads are formed into a non-woven tissue.

11. Implant according to claim 8, wherein the implant comprises adherent cells.

12. Implant according to claim 11, wherein the cells are obtained from the future recipient of the implant.

13. Implant according to claim 8, wherein the spider silk threads have a length in the range of 0.1 mm to 200 mm.

14. Implant for medical use consisting of a scaffolding matrix consisting of cross-linked spider silk threads moulded into a pre-determined three-dimensional form with only a cross-linking agent.