Oxidizable species as an internal reference for biosensors and method of use
First Claim
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1. A biosensor for determination of an analyte concentration in a test sample comprising:
- a working electrode and a counter electrode;
a mixture for electrochemical reaction with an analyte, said mixture including an enzyme, a mediator, and an oxidizable species as an internal reference, the oxidizable species being different than the mediator species and having different redox potentials, said mixture being located as a single layer directly on a surface of at least a portion of the working electrode and the counter electrode prior to the introduction of the test sample, wherein the mixture includes only one enzyme.
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Abstract
A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.
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Citations
23 Claims
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1. A biosensor for determination of an analyte concentration in a test sample comprising:
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a working electrode and a counter electrode; a mixture for electrochemical reaction with an analyte, said mixture including an enzyme, a mediator, and an oxidizable species as an internal reference, the oxidizable species being different than the mediator species and having different redox potentials, said mixture being located as a single layer directly on a surface of at least a portion of the working electrode and the counter electrode prior to the introduction of the test sample, wherein the mixture includes only one enzyme. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of forming and applying a reagent mixture for an electrochemical reaction with an analyte of a fluid sample in a biosensor, the biosensor having a working electrode and a counter electrode, the method comprising:
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forming a batch of reagent mixture by adding an enzyme, adding a mediator and adding a known amount of an oxidizable species, the added oxidizable species being added separately from the mediator; and after forming the reagent mixture, placing the reagent mixture directly on a surface of at least a portion of the working electrode and the counter electrode of the biosensor prior to the introduction of the fluid sample, wherein the mixture includes only one enzyme, the oxidizable species is different than the mediator species and the mediator and the oxidizable species have different redox potentials. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A biosensor for determination of an analyte concentration in a test sample comprising:
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a working electrode and a counter electrode; a mixture for electrochemical reaction with an analyte, said mixture including an enzyme, a mediator, and an oxidizable species as an internal reference, the oxidizable species being different than the mediator species and having different redox potentials, said mixture being located as a single layer directly on a surface of at least a portion of the working electrode prior to the introduction of the test sample, wherein the mixture includes only one enzyme. - View Dependent Claims (22, 23)
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Specification