Mediator stabilized reagent compositions for use in biosensor electrodes
First Claim
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1. A method of determining concentration of an analyte, the method comprising:
- contacting a sample with a biosensor strip comprising;
an electrode support;
a working electrode disposed on the electrode support;
a reference electrode disposed on the electrode support;
a reagent composition deposited on at least one of the working electrode and the reference electrode, wherein the reagent composition comprises;
an enzyme, anda redox mediator complex comprising;
a first metal ion covalently bonded to at least one compound comprising a 1,2-quinone moiety; and
a second metal ion non-covalently bonded to the oxygen atoms of the 1,2-quinone moiety;
applying a potential to the working electrode;
measuring a current from the working electrode; and
determining the analyte concentration.
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Abstract
The claimed subject matter relates to the stabilization of 1,2-quinone mediators, especially those containing 1,10-phenanthroline quinone (PQ) and more especially transition metal complexes of PQ, in the presence of enzymes when contained in dry reagent layers for biosensor electrodes, through the use of various metal salts, particularly those of lithium.
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Citations
16 Claims
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1. A method of determining concentration of an analyte, the method comprising:
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contacting a sample with a biosensor strip comprising; an electrode support; a working electrode disposed on the electrode support; a reference electrode disposed on the electrode support; a reagent composition deposited on at least one of the working electrode and the reference electrode, wherein the reagent composition comprises; an enzyme, and a redox mediator complex comprising; a first metal ion covalently bonded to at least one compound comprising a 1,2-quinone moiety; and a second metal ion non-covalently bonded to the oxygen atoms of the 1,2-quinone moiety; applying a potential to the working electrode; measuring a current from the working electrode; and determining the analyte concentration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification