Sampling device with immiscible fluid supply tube in counter-flow arrangement

• US 8,697,011 B2
• Filed: 05/19/2009
• Issued: 04/15/2014
• Est. Priority Date: 05/19/2009
• Status: Active Grant

• Current Assignee: Stokes Bio Ltd. (Perspecta, Inc.)
• Original Assignee: Stokes Bio Ltd. (Perspecta, Inc.)
• Inventors: Curran, Kieran, McGuire, David, Garvey, Julie, Curtain, Damian
• Primary Examiner(s): HIXSON, CHRISTOPHER
• Application Number:
  US12/468,367
• Publication Number:
  US 20100294048A1
• Time in Patent Office: 1,792 Days
• Field of Search: None
• US Class Current: 422/510
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]

Abstract

The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including: a sampling member for acquiring or dispensing a sample; and a supply of a fluid that is immiscible with the sample; in which the device is configured to provide a continuous flow of immiscible fluid enveloping the sampling member.

Claims

1. A sampling device comprising:

an outer sheath comprising a distal end on a lower portion of the outer sheath;

a plurality of tubes within the outer sheath, the plurality comprising at least one immiscible fluid supply tube;

at least one actuatable sample tube comprising a distal end proximate the lower portion of the outer sheath, wherein the at least one actuatable sample tube is within the outer sheath and is substantially parallel to the plurality of tubes within the outer sheath;

at least one pump in operable communication with the at least one actuatable sample tube, the at least one immiscible fluid supply tube, or both;

at least one immiscible fluid supply source in fluid communication with the at least one immiscible fluid supply tube; and

at least one actuator operably associated with the at least one actuatable sample tube and configured to extend the distal end of the at least one actuatable sample tube beyond the distal end of the outer sheath and retract the distal end of the at least one actuatable sample tube to within the outer sheath,

wherein the device is configured to provide counter-flow between the immiscible fluid supply tube and the at least one actuatable sample tube.

2. The device according to claim 1, wherein the at least one sample tube and at least one immiscible fluid supply tube are in fluid communication with one another and the device is configured to provide a counter-flow of an immiscible fluid between the immiscible fluid supply tube and the at least one actuatable sample tube.

3. The device according to claim 2, wherein the at least one immiscible fluid supply tube is configured to continuously expel immiscible fluid from the immiscible fluid supply source.

4. The device according to claim 3, wherein the at least one actuatable sample tube is configured to envelope the sample within the immiscible fluid.

5. The device according to claim 1, wherein the at least one actuatable sample tube is configured to acquire a sample without the introduction of a non-dissolved gas into the at least one actuatable sample tube that acquires the sample.

6. The device according to claim 1, wherein the device is in fluid communication with a liquid bridge system.

7. A system for sample acquisition comprising:

a sampling device comprising

an outer sheath comprising a distal end on a lower portion of the outer sheath;

a plurality of tubes within the outer sheath, the plurality of tubes comprising at least one immiscible fluid supply tube; and

at least one actuatable sample tube having a distal end proximate the lower portion of the outer sheath, wherein the at least one actuatable sample tube is within the outer sheath and is substantially parallel to the plurality of tubes within the outer sheath;

at least one pump in operable communication with the at least one actuatable sample tube, the at least one immiscible fluid supply tube, or both;

at least one immiscible fluid supply source in fluid communication with the at least one immiscible fluid supply tube; and

at least one actuator operably associated with the at least one actuatable sample tube and configured to extend tile distal end of the at least one actuatable sample tube beyond tile distal end of the outer sheath and retract the distal end of the at least one actuatable sample tube to be within the outer sheath;

at least one vessel configured for containment of a sample and all overlay of a fluid that is immiscible with the sample; and

wherein the distal end of the outer sheath and the distal end of the at least one actuatable sample tube are configured to interact with the at least one vessel to acquire the sample without also acquiring a non-dissolved gas,

wherein the device is configured to provide counter-flow between the immiscible fluid supply tube and the at least one actuatable sample tube.

8. The system according to claim 7, wherein a counter-flow exists between the at least one actuatable sample tube and the at least one immiscible fluid and the tube that acquires the sample supply tube.

9. The system according to claim 8, wherein the at least one immiscible fluid supply tube is configured to continuously expel the immiscible fluid.

10. The system according to claim 9, wherein the at least one actuatable sample tube is configured to envelope a sample within the immiscible fluid.

11. The system according to claim 7, further comprising at least one robotics system configured to control movement of the sampling device, and configured to control movement of the at least one actuatable sample tube.

12. The system according to claim 11, wherein the at least one pump comprises a first pump connected to the at least one actuatable sample tube, and a second pump connected to the at least one immiscible fluid supply tube that.

13. The system according to claim 7, further comprising a liquid bridge that is in fluid contact communication with continuously the sampling device.