Detection of phage amplification by SERS nanoparticles
First Claim
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1. A lateral flow device for detection of phage amplification comprising:
- at least one phage specific antibody presenting particle attached to at least one lateral flow strip; and
a Raman spectrometer operably associated with the at least one lateral flow strip to identify said particle to detect phage amplification, wherein the at least one lateral flow strip is configured to be detected by a single Raman source and wherein the phage specific antibody presenting particle comprises;
a coating comprising at least one reporter molecule attached to the surface of a metal nanoparticle core;
a glass coating attached to the surface of said coating; and
a phage specific antibody attached to the surface of said glass coating.
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Abstract
A phage specific antibody presenting particle, devices and methods related to detection of phage amplification are provided.
87 Citations
14 Claims
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1. A lateral flow device for detection of phage amplification comprising:
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at least one phage specific antibody presenting particle attached to at least one lateral flow strip; and a Raman spectrometer operably associated with the at least one lateral flow strip to identify said particle to detect phage amplification, wherein the at least one lateral flow strip is configured to be detected by a single Raman source and wherein the phage specific antibody presenting particle comprises; a coating comprising at least one reporter molecule attached to the surface of a metal nanoparticle core; a glass coating attached to the surface of said coating; and a phage specific antibody attached to the surface of said glass coating. - View Dependent Claims (2, 3, 4)
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5. A lateral flow device for multiplexing analysis comprising:
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at least one lateral flow strip; at least one phage specific antibody presenting particle with a first type of reporter molecule coating and at least one phage specific antibody presenting particle with a second type of reporter molecule coating operably associated with said at least one lateral flow strip; and a Raman spectrometer operably associated with said at least one lateral flow strip to identify said particle to detect phage amplification, wherein the strip is arranged so it can be detected by a single Raman source, and wherein the phage specific antibody presenting particle comprises; a coating comprising at least one reporter molecule attached to the surface of a metal nanoparticle core; a glass coating attached to the surface of said coating; and a phage specific antibody attached to the surface of said glass coating. - View Dependent Claims (6, 7, 8, 9)
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10. A lateral flow device for detection of phage amplification comprising:
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a particle comprising a phage specific antibody presenting particle and a first phage specific antibody attached to the particle; at least one amplified phage conjugated to said particle; and a solid support comprising; a stationary phase; and a second phage specific antibody immobilized on said stationary phase, wherein said second antibody binds to said at least one of said phage, and wherein the phage specific antibody presenting particle comprises; a coating comprising at least one reporter molecule attached to the surface of a metal nanoparticle core; a glass coating attached to the surface of said coating; and a phaqe specific antibody attached to the surface of said glass coating. - View Dependent Claims (11, 12, 13)
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14. A method of detecting phage amplification comprising:
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adding a progeny phage to at least one lateral flow strip, wherein the lateral flow strip includes at least one phage specific antibody immobilized to said lateral flow strip; contacting the progeny phage with at least one phage specific antibody presenting particle to create a phage-particle complex, wherein the phage specific antibody presenting particle comprises; a coating comprising at least one reporter molecule attached to the surface of a metal nanoparticle core; a glass coating attached to the surface of said coating; and a phage specific antibody attached to the surface of said glass coating; interrogating the lateral flow strip with a Raman spectrometer; and identifying said phage-particle complex to detect phage amplification.
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Specification