Generating artificial hyperspectral images using correlated analysis of co-registered images
First Claim
1. A method comprising:
- (a) defining a first tile on a first digital image of a first slice of a tissue sample, wherein the first tile has a first position in the first digital image;
(b) defining a second tile on a second digital image of a second slice of the tissue sample, wherein the second tile has a second position in the second digital image, and wherein the second slice originated from the tissue sample adjacent to the first slice;
(c) calculating a first value associated with image objects detected in the first tile;
(d) calculating a second value associated with image objects detected in the second tile;
(e) generating a first hyperspectral image using the first digital image, wherein a first pixel of the first hyperspectral image has a first pixel value corresponding to the first value and a first pixel location corresponding to the first position;
(f) generating a second hyperspectral image using the second digital image, wherein a second pixel of the second hyperspectral image has a second pixel value corresponding to the second value and a second pixel location corresponding to the second position;
(g) generating a third hyperspectral image using the first hyperspectral image and the second hyperspectral image; and
(h) displaying the third hyperspectral image on a computer monitor using a false-color encoding generated using the first pixel value and the second pixel value.
1 Assignment
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Accused Products
Abstract
High-resolution digital images of adjacent slices of a tissue sample are acquired, and tiles are defined in the images. Values associated with image objects detected in each tile are calculated. The tiles in adjacent images are co-registered. A first hyperspectral image is generated using a first image, and a second hyperspectral image is generated using a second image. A first pixel of the first hyperspectral image has a first pixel value corresponding to a local value obtained using image analysis on a tile in the first image. A second pixel of the second hyperspectral image has a second pixel value corresponding to a local value calculated from a tile in the second image. A third hyperspectral image is generated by combining the first and second hyperspectral images. The third hyperspectral image is then displayed on a computer monitor using a false-color encoding generated using the first and second pixel values.
20 Citations
21 Claims
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1. A method comprising:
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(a) defining a first tile on a first digital image of a first slice of a tissue sample, wherein the first tile has a first position in the first digital image; (b) defining a second tile on a second digital image of a second slice of the tissue sample, wherein the second tile has a second position in the second digital image, and wherein the second slice originated from the tissue sample adjacent to the first slice; (c) calculating a first value associated with image objects detected in the first tile; (d) calculating a second value associated with image objects detected in the second tile; (e) generating a first hyperspectral image using the first digital image, wherein a first pixel of the first hyperspectral image has a first pixel value corresponding to the first value and a first pixel location corresponding to the first position; (f) generating a second hyperspectral image using the second digital image, wherein a second pixel of the second hyperspectral image has a second pixel value corresponding to the second value and a second pixel location corresponding to the second position; (g) generating a third hyperspectral image using the first hyperspectral image and the second hyperspectral image; and (h) displaying the third hyperspectral image on a computer monitor using a false-color encoding generated using the first pixel value and the second pixel value. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification