Method for operating a measurement for a sample on an electrochemical test strip

• US 8,702,960 B2
• Filed: 10/04/2011
• Issued: 04/22/2014
• Est. Priority Date: 07/06/2011
• Status: Active Grant

• Current Assignee: Bionime Corporation
• Original Assignee: Bionime Corporation
• Inventors: Hsu, Cheng-Teng, Su, Chun-Wei, Chen, Chieh-Hsing
• Primary Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US13/252,973
• Publication Number:
  US 20130008802A1
• Time in Patent Office: 931 Days
• Field of Search: 205/775, 205/777.5, 205/778, 205/792, 20440301-40315, 600345-348, 422/68.1, 422/82.01, 435/287.1, 435/287.2
• US Class Current: 205/777.5
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771

Abstract

A method for operating a measurement for a sample on an electrochemical test strip including at least two electrodes is provided. The method includes steps of applying a first voltage between the two electrodes during an interference-removal period after an incubation period succeeding a moment when the sample is detected, and applying a second voltage between the two electrodes during a test period, wherein the first voltage is larger than the second voltage, the first voltage includes one of a first fixed voltage and a first set of plural pulse voltages, and the second voltage includes a second fixed voltage.

Claims

1. A method for operating a measurement for a sample on an electrochemical test strip, wherein the sample includes an interference material and an analyte concentration, the method comprising steps of:

providing the electrochemical test strip, wherein the electrochemical test strip includes two electrodes, a hydrogen peroxide and a reagent containing a mediator having a reduction state and an oxidation state;

applying a detection voltage between the two electrodes during a sample presence detection period;

applying an incubation voltage between the two electrodes during an incubation period succeeding a moment when the electrochemical test strip is detected to have the sample thereon, wherein;

the incubation voltage has a maximum voltage value,

the maximum voltage value is high enough to oxidize the mediator from the reduction state to the oxidation state and is low enough to avoid oxidizing the hydrogen peroxide,

the incubation voltage includes an incubation set of plural pulse voltages, each of which is in a range of 0.1-0.6 volt,

each pulse of the incubation set of plural pulse voltages has an incubation pulse width in a range of 100-500 μ

s, and

the incubation set of plural pulse voltages has an incubation pulse interval between two adjacent pulses therein, wherein the incubation pulse interval is in a range of 100-500 ms;

oxidizing the interference material by applying a first voltage between the two electrodes during an interference-removal period after the incubation period, wherein the first voltage includes one of a first fixed voltage and a first set of plural pulse voltages and is in a range of 0.15-1 volt, and the first voltage is applied for 0.1-1 s;

measuring the analyte concentration by applying a second voltage between the two electrodes during a test period, wherein the first voltage is higher than the second voltage, the second voltage includes a second fixed voltage and is in a range of 0.1-0.9 volt, and the second voltage is applied for 0.2-10 s; and

during a transition interval between the interference-removal period and the test period, performing one of following operations;

causing a power-off to result in an open-circuit state in a measuring circuit; and

applying a zero voltage between the two electrodes, wherein the transition interval lasts 0.01-0.5 s.

2. A method of claim 1, wherein

the step of applying the detection voltage continues till the sample is detected;

when a detected electrical current between the two electrodes is higher than a threshold, the electrochemical test strip is deemed to have the sample thereon, and the sample presence detection period is ended;

the detection voltage includes a detection set of plural pulse voltages, each of which is in a range of 0.1-0.5 volt;

each pulse of the detection set of plural pulse voltages has a detection pulse width in a range of 100-600 μ

s; and

the detection set of plural pulse voltages has a detection pulse interval between two adjacent pulses therein, wherein the detection pulse interval is in a range of 10-50 ms.

3. A method of claim 2, wherein:

the sample includes a blood sample;

the each voltage of the detection set of plural pulse voltages is in a range of 0.15-0.25 volt;

the detection pulse width is in a range of 300-400 μ

s; and

the detection pulse interval is in a range of 15-25 ms.

4. A method of claim 1, wherein:

the each voltage of the incubation set of plural pulse voltages is in a range of 0.15-0.25 volt;

the incubation pulse width is in a range of 150-250 μ

s;

the incubation pulse interval is in a range of 150-250 ms;

the reagent further includes a glucose oxidase enzyme; and

the mediator includes a potassium ferrocyanide.

5. A method of claim 1, wherein:

the sample includes a blood sample; and

the second voltage is applied to measure an electrical parameter, thereby measuring the analyte concentration.

6. A method of claim 5, wherein:

the electrical parameter includes a detected electrical current between the two electrodes;

the detected electrical current and the analyte concentration have a mathematical linear relationship in a measuring domain;

the analyte concentration is calculated based on the detected electrical current; and

the analyte concentration includes a blood sugar concentration.