Moving microdroplets in a microfluidic device
DCFirst Claim
1. A method of amplifying a nucleic acid-containing sample within a microfluidic device, the method comprising:
- moving the sample from an upstream channel of the microfluidic device into a DNA manipulation module located downstream of the upstream channel, the DNA manipulation module including a DNA manipulation zone configured to perform amplification of the sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone, the only ingress to and egress from the DNA manipulation zone being through the first valve and the second valve;
receiving the sample in the DNA manipulation zone;
closing the first valve and the second valve such that gas and liquid are prevented from flowing into or out of the DNA manipulation zone; and
thermal cycling the sample in the DNA manipulation zone.
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Abstract
This disclosure provides systems, methods, and devices for processing samples on a microfluidic device. One method includes moving a sample from an upstream channel of a microfluidic device into a DNA manipulation module located downstream of the upstream channel. The DNA manipulation module includes a DNA manipulation zone configured to perform amplification of the sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone. The method also includes receiving the sample in the DNA manipulation zone; closing the first valve and the second valve such that as and liquid are prevented from flowing into or out of the DNA manipulation zone; and thermal cycling the sample in the DNA manipulation zone.
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Citations
10 Claims
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1. A method of amplifying a nucleic acid-containing sample within a microfluidic device, the method comprising:
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moving the sample from an upstream channel of the microfluidic device into a DNA manipulation module located downstream of the upstream channel, the DNA manipulation module including a DNA manipulation zone configured to perform amplification of the sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone, the only ingress to and egress from the DNA manipulation zone being through the first valve and the second valve; receiving the sample in the DNA manipulation zone; closing the first valve and the second valve such that gas and liquid are prevented from flowing into or out of the DNA manipulation zone; and thermal cycling the sample in the DNA manipulation zone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification