Cell culture vessels for meniscus reduction with aqueous solutions
First Claim
Patent Images
1. A meniscus reducing member comprising:
- a) a vessel, the vessel comprising a top portion, a closed bottom wall and a sidewall extending from the bottom wall for retaining a given volume of liquid within the vessel, the sidewall comprising an interior surface; and
b) a surface feature overlying at least a portion of the interior surface of the vessel, the surface feature includes at least two surfaces for contacting the liquid, the at least two surfaces cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature, the at least two surfaces comprise a plurality of alternating first and second surface regions, each first surface region having a first surface energy and each second surface region having a second surface energy, the second surface energy being different than the first surface energy and the first surface region has a relatively lower degree of hydrophobicity than the second surface region.
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Abstract
A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
22 Citations
13 Claims
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1. A meniscus reducing member comprising:
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a) a vessel, the vessel comprising a top portion, a closed bottom wall and a sidewall extending from the bottom wall for retaining a given volume of liquid within the vessel, the sidewall comprising an interior surface; and b) a surface feature overlying at least a portion of the interior surface of the vessel, the surface feature includes at least two surfaces for contacting the liquid, the at least two surfaces cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature, the at least two surfaces comprise a plurality of alternating first and second surface regions, each first surface region having a first surface energy and each second surface region having a second surface energy, the second surface energy being different than the first surface energy and the first surface region has a relatively lower degree of hydrophobicity than the second surface region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of preparing a liquid for visual observation, the method comprising:
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a) placing liquid into a vessel, the vessel comprising a surface feature on an interior surface of the vessel, the surface feature comprising at least two surfaces that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature, the at least two surfaces comprise a plurality of alternating first and second surface regions, each first surface region having a first surface energy and each second surface region having a second surface energy, the second surface energy being different than the first surface energy and the first surface region has a relatively lower degree of hydrophobicity than the second surface region; b) filling the vessel with a given volume of the liquid until the free surface of the liquid contacts the surface feature to reduce the width of the meniscus formed at the interface between the free surface of the liquid and the surface feature; and c) retaining the volume of liquid in the vessel for observation.
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Specification