Methods of determining NOx in a wound sample

• US 8,703,101 B2
• Filed: 08/03/2010
• Issued: 04/22/2014
• Est. Priority Date: 08/04/2009
• Status: Active Grant

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Bernatchez, Stephanie F., Menon, Vinod P., Stoffel, Joseph J., Tucker, Joseph A.
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): Knight, Teresa E
• Application Number:
  US13/388,360
• Publication Number:
  US 20120135444A1
• Time in Patent Office: 1,358 Days
• Field of Search: None
• US Class Current: 424/9.1
• CPC Class Codes: G01N 2800/40 Disorders due to exposure to physical agents, e.g. heat disorders, motion sickness, radiation injuries, altitude sickness, decompression illness
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/177692 Oxides of nitrogen

Abstract

The present disclosure provides devices and rapid methods to acquire a wound sample to detect and measure NOx, and optionally, one or more other analytes that are indicative of the status of a wound.

Claims

1. A method comprising:

providing a sample acquisition device comprising an absorbent pad that comprises less than 31 ng of reactive nitrates per cm²;

contacting the sample acquisition device with a wound site for about 24 hours or less;

extracting a portion of the exudate from the absorbent pad of the sample acquisition device; and

detecting endogenous NOx in the extracted portion.

2. The method of claim 1, further comprising detecting in at least one extracted portion at least one analyte in addition to endogenous NO_x.

3. The method of claim 2, wherein detecting in the extracted portion at least one analyte in addition to endogenous NO_x comprises detecting total protein.

4. The method of claim 3, wherein detecting total protein comprises detecting protein colorimetrically.

5. The method of claim 2, wherein detecting in the extracted portion at least one analyte in addition to endogenous NO_x comprises detecting a protease.

6. The method of claim 2, wherein detecting at least one analyte in addition to endogenous NOx comprises detecting an analyte from the group consisting of:

detecting total protein, a protein or a fragment thereof, a protease, a polynucleotide, a cytokine, a growth factor, a microorganism or an analyte therefrom, or a combination of any two or more of the foregoing analytes.

7. The method of claim 2, wherein detecting in the extracted portion at least one analyte in addition to endogenous NOx comprises detecting total protein and matrix metalloproteinase MMP-9.

8. The method of claim 1, wherein detecting endogenous NO_x comprises detecting NO_x by spectrometry.

9. The method of claim 8, wherein detecting NO_x by spectrometry comprises detecting NO_x colorimetrically.

10. The method of claim 8, wherein detecting NO_x by spectrometry comprises detecting NO_x fluorometrically.

11. The method of claim 1, wherein extracting the exudate comprises centrifuging the sample acquisition device.

12. The method of claim 1, further comprising the step of measuring the pH of the wound.

13. The method of claim 1, further comprising cleansing the wound.

14. The method of claim 1, wherein contacting the sample acquisition device with a wound site for a period of time comprises manually pressing the device against the wound site.

15. The method of claim 1, wherein contacting the sample acquisition device with a wound comprises contacting the sample acquisition device with a wound site for less than 30 minutes.

16. The method of claim 15, wherein contacting the sample acquisition device with a wound comprises contacting the sample acquisition device with a wound site for less than 1 minute.

17. The method of claim 1, wherein, after the sample acquisition device is contacted with the wound site, the sample acquisition device is frozen.

18. A method comprising:

providing at least two sample acquisition devices, each sample acquisition device comprising an absorbent pad that comprises less than 31 ng of reactive nitrates per cm²;

contacting a first sample acquisition device with a wound site for about 24 hours or less;

cleansing the wound site;

contacting a second sample acquisition device with the wound site for about 24 hours or less;

extracting a portion of the exudate from the absorbent pad of each of the first and second sample acquisition devices; and

detecting endogenous NOx in the extracted portion from each sample acquisition device.