Molecular diagnostics amplification system and methods

• US 8,703,445 B2
• Filed: 12/15/2006
• Issued: 04/22/2014
• Est. Priority Date: 12/29/2005
• Status: Expired due to Fees

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Collier, Gordon Bruce, MacLeod, Jason Andrew, Nemeth, Attila Csaba, Dicke, William Charles
• Primary Examiner(s): MARSCHEL, ARDIN H
• Application Number:
  US11/611,677
• Publication Number:
  US 20070154922A1
• Time in Patent Office: 2,685 Days
• Field of Search: 422/50, 422/417, 422/420, 422/430, 422/68.1, 422/69, 422/500, 422/502, 422/129, 422/131, 422/138, 435/6.1, 435/6.11, 435/6.12, 435/91.2, 435/283.1, 435/289.1
• US Class Current: 435/91.2
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/5029 using swabs
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

The present invention relates to automated devices and methods for the amplification of segments of nucleic acid in a convenient and portable manner. A single-use nucleic acid amplification device for producing an amplicon includes a housing and an amplification chamber. The chamber includes an ingress with a first reversible seal, an egress with a second reversible seal, a sealable sample entry orifice, and a first wall forming a portion of the chamber. The first wall includes a thermally conductive material and includes an interior surface and an exterior surface. The exterior surface includes a heating circuit and a temperature sensor. The sample entry orifice permits a sample of nucleic acid to enter the amplification chamber. The ingress is connected to a first conduit along with a pneumatic pump and a fluid pouch. The egress is connected to a second conduit permitting egress of the amplicon from the amplification chamber.

Claims

1. A nucleic acid amplification device comprising:

a housing; and

an amplification chamber comprising;

an ingress with a first reversible seal;

an egress with a second reversible seal;

a sealable sample entry orifice; and

a first wall forming a portion of the amplification chamber,

wherein the first wall comprises a thermally conductive material and includes a first surface and a second surface,

wherein the second surface includes a heating circuit and a temperature sensor,

wherein the sample entry orifice permits a sample of nucleic acid to enter the amplification chamber,

wherein the ingress is connected to a first conduit along with a reservoir comprising a first flexible diaphragm and a fluid pouch for performing nucleic acid amplification, and a pump comprising a pneumatic pump and a second flexible diaphragm,

wherein the first flexible diaphragm is configured to engage and be actuated by a plunger on an instrument with which the amplification device is configured to mate,

wherein the second flexible diaphragm is configured to engage and be actuated by a plunger on an instrument with which the amplification device is configured to mate, and

wherein the egress is connected to a second conduit permitting egress of an amplicon from the amplification chamber.

2. The amplification device of claim 1, wherein the first wall comprises silicon and wherein the silicon comprises about 30 to about 50 percent of the first surface area of the amplification chamber.

3. The amplification device of claim 2, wherein the sample entry orifice is configured to mate with a sample introduction element comprising a wand.

4. The amplification device of claim 1, wherein the amplification chamber includes a second wall comprising a plastic material.

5. The amplification device of claim 4, wherein the second wall comprises a wall thickness in the range of about 0.2 mm to about 5 mm, and wherein the second wall includes one or more additional rib supports.

6. The amplification device of claim 1, wherein an internal volume of the amplification chamber is in the range of about 5 uL to about 50 uL.

7. The amplification device of claim 1, wherein an amplification chamber surface to an amplification chamber volume ratio is in the range of about 50 to about 200 square mm for the amplification chamber surface and to about 5 to about 30 cubic mm for the amplification chamber volume.

8. The amplification device of claim 1, wherein an internal shape of the amplification chamber comprises one of a substantially rectangular structure, a substantially rectangular shape with rounded corners, a cylinder, and a cylindrical structure with a substantially oval cross-section.

9. The amplification device of claim 1, wherein the second surface of the first wall comprises a heating circuit.

10. The amplification device of claim 9, wherein the heating circuit comprises a resistive electrical path fabricated on the second surface with a first and second connecting pad for contacting an external circuit for providing current flow through the path.

11. The amplification device of claim 1, wherein the second surface of the first wall comprises a temperature sensor and wherein the temperature sensor comprises one of a thermistor and a thermocouple fabricated on the second surface with a first and second connecting pad for contacting an external circuit for connecting to the one of the thermistor and the thermocouple.

12. The amplification device of claim 1, wherein the amplification chamber comprises a sugar glass coating on at least a portion of the first surface of the first wall.

13. The amplification device of claim 1, wherein the amplification chamber is configured to have a temperature increase ramp rate in the range of about 10 to about 50 degrees centigrade per second.

14. The amplification device of claim 1, wherein the amplification chamber is configured to have a temperature decrease ramp rate in the range of about 4 to about 50 degrees centigrade per second.

15. The amplification device of claim 1, wherein the amplification chamber comprises an optical window.

16. The amplification device of claim 1, wherein the second surface of the first wall comprises a Peltier circuit with a first and second connecting pad for contacting an external circuit.

17. The amplification device of claim 1, wherein the first reversible seal comprises a flexible diaphragm.

18. The amplification device of claim 1, wherein the second conduit comprises a mating feature for engaging a device for detection of the amplicon.

19. The amplification device of claim 1, wherein the first conduit comprises a chip insert with a fluid detection sensor.