Cartridge for automated medical diagnostics

• US 8,703,476 B2
• Filed: 06/26/2006
• Issued: 04/22/2014
• Est. Priority Date: 06/30/2005
• Status: Active Grant

• Current Assignee: Biocartis NV (Biocartis Group NV)
• Original Assignee: Biocartis SA (Biocartis Group NV)
• Inventors: De Gier, Ronald Cornelis, Schaefer, Danny Genius Aldegonda, Van Den Bijgaart, Adrianus Wilhelmus Dionisius Maria, Van Haag, Chris, De Jong, Michiel
• Primary Examiner(s): Beisner, William H
• Assistant Examiner(s): HENKEL, DANIELLE B
• Application Number:
  US11/993,348
• Publication Number:
  US 20100151565A1
• Time in Patent Office: 2,857 Days
• Field of Search: 435/287.2, 435/289.1, 435/91.2, 435/287.3, 422/68.1, 422/50, 436/165
• US Class Current: 435/289.1
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

The present invention relates to a cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a first component and a second component being connectable to each other, the first component comprising at least a first fluid opening and a first sealing surface and the second component comprising at least a second fluid opening and a second sealing surface. Upon connection of the first and second component the first and second fluid opening are placeable in fluid communication and the first and second sealing surfaces are placeable against each other to seal the fluid communication between the first and second fluid opening. The invention is characterized in that the cartridge comprises biasing means for biasing the second sealing surface in the direction of the first sealing surface.

Claims

1. A cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences, the cartridge comprising:

a first component for processing a fluid having a sample, said first component including;

one or more processing chambers,

a first fluid opening for fluid access to at least one of said processing chambers of the first component, and

a first sealing surface associated with the first fluid opening;

a second component connectable to the first component for processing the fluid, said second component including;

one or more processing chambers,

a second fluid opening for fluid access to at least one of said processing chambers of the second component, said second fluid opening formed in a flexible portion of the second component that is at least flexible in a direction perpendicular to the second sealing surface, and

a second sealing surface associated with the second fluid opening,

the first and second components are moveable relative to each other when the first component is connected to the second component to selectively position the first and second fluid openings in (i) an open fluid communication position, wherein the first fluid opening is generally aligned with the second fluid opening to allow the fluid to move between the first component and the second component, and (ii) a closed fluid communication position, wherein the first fluid opening is unaligned with the second fluid opening to prevent the fluid from moving between the first component and the second component; and

a biaser for biasing the second sealing surface toward the first sealing surface to engage the second sealing surface with the first sealing surface to provide a sealed fluid pathway between the first and second components, thereby preventing fluid from leaking external to said cartridge.

2. A cartridge according to claim 1, wherein the second component comprises two or more flexible portions, each having a second fluid opening and an associated second sealing surface and each being at least flexible in a direction perpendicular to the respective second sealing surface.

3. A cartridge according to claim 1, wherein the first and second sealing surfaces are substantially flat, the planes of each of the first and second sealing surfaces being substantially parallel to each other.

4. A cartridge according to claim 1, wherein the second component is disk shaped and comprises a ring-shaped main body and a plurality of fingerlike flexible parts, one end of each flexible part being connected to the main body and an opposite end of the flexible part being free, wherein the second fluid opening and the second sealing surface are provided at the opposite end of the flexible part.

5. A cartridge according to claim 1, wherein the first component is a main part of the cartridge, and the second component is a PCR body comprising said one or more processing chambers.

6. A cartridge according to claim 5, wherein said second fluid opening is an inlet and outlet for one of the one or more processing chambers of the second component.

7. A cartridge according to claim 4 wherein each of the one or more processing chambers of the second component are formed by a space between the main body and a flexible foil joined to the main body at the edges of the space, the second fluid opening being in fluid communication with the space.

8. A cartridge according to claim 1, wherein the biaser is comprised of a locking device to lock the second component to the first component.

9. A cartridge according to claim 8, wherein the locking device comprises at least one spring element to bias the second sealing surface in the direction of the first sealing surface.

10. A cartridge according to claim 9, wherein after connection of the first component to the second component, the first and second components are rotatable relative to each other around an axis of rotation, and wherein the first and second component each comprise two or more fluid openings being substantially arranged in a circle around the axis of rotation.

11. A cartridge according to claim 1, wherein at least one of the first or second sealing surfaces is made of a relative soft material.

12. A cartridge according to claim 1, wherein at least one of the first and second components is provided with a seal element comprising the first fluid opening and first sealing surface or the second fluid opening and second sealing surface, respectively, the seal element being configured as a valve to provide in the open fluid communication position a fluid communication between the first and second fluid openings, and to provide in the closed fluid communication position an obstruction of the fluid communication, the seal element further being configured to seal the first and second fluid openings from the environment in both the open and closed fluid communication positions.

13. A cartridge according to claim 8, wherein the locking device is configured to be used to move the second component between a first and second position.

14. A cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences, the cartridge comprising:

a first component for processing a fluid having a sample, said first component including;

one or more processing chambers,

a first fluid opening for fluid access to at least one of said processing chambers of the first component, and

a first sealing surface associated with the first fluid opening;

a second component connectable to the first component for processing the fluid, said second component including;

a disk shaped body including at least one flexible portion,

one or more processing chambers located within the disk shaped body, a second fluid opening formed in the at least one flexible portion, the second fluid opening providing fluid access to at least one of said processing chambers of the second component, and

a second sealing surface associated with the second fluid opening,

the first and second components moveable relative to each other when the first component is connected to the second component, for selectively positioning the first and second fluid openings in (i) an open fluid communication position, wherein the first fluid opening is generally aligned with the second fluid opening to allow the fluid to move between the first component and the second component, and (ii) a closed fluid communication position, wherein the first fluid opening is unaligned with the second fluid opening to prevent the fluid from moving between the first component and the second component; and

a biaser for biasing the second sealing surface toward the first sealing surface to engage the second sealing surface with the first sealing surface, wherein engagement of said first sealing surface with said second sealing surface provides a sealed fluid pathway between the first and second components, thereby preventing the fluid from leaking external to said cartridge.

15. The cartridge according to claim 14, wherein upon connection of the first component to the second component, the first and second components are rotatable relative to each other.

16. The cartridge according to claim 14, wherein being positioned to provide fluid communication between the first and second fluid openings, the second sealing surface is structured and arranged to be positionable in relation to the first sealing surface to close the fluid communication.

17. The cartridge according to claim 14, further comprising a locking device postionable to lock the second component to the first component, the locking device comprising the biaser.

18. The cartridge according to claim 17, wherein the biaser comprises at least one spring element to bias the second sealing surface in the direction of the first sealing surface.