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Methods and compositions for targeted single-stranded cleavage and targeted integration

  • US 8,703,489 B2
  • Filed: 08/18/2009
  • Issued: 04/22/2014
  • Est. Priority Date: 08/22/2008
  • Status: Active Grant
First Claim
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1. A protein complex comprising:

  • (a) a first fusion protein comprising a DNA-binding domain and at least one catalytically inactive FokI cleavage domain or cleavage half-domain, wherein the cleavage domain or cleavage half-domain comprises a mutation at one or more of amino acid residues 450, 467 and 469; and

    (b) a second fusion protein comprising a zinc finger domain and a FokI catalytically active cleavage half domain,wherein the catalytically inactive FokI cleavage half domain forms a heterodimer with the catalytically active FokI cleavage half-domain of the second fusion protein and further wherein the protein complex induces a single-stranded break in a target double-stranded sequence.

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