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Membrane-based lateral flow assay devices that utilize phosphorescent detection

  • US 8,703,504 B2
  • Filed: 09/04/2013
  • Issued: 04/22/2014
  • Est. Priority Date: 11/21/2003
  • Status: Expired due to Term
First Claim
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1. A method for detecting an analyte within a test sample, the method comprising:

  • i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with detection probes, the detection probes comprising a phosphorescent particle, the phosphorescent particle comprising a phosphorescent metal complex encapsulated within a barrier matrix comprising polymethylmethacrylate, the detection probes being conjugated with a specific binding member configured to bind with the analyte and form an analyte complex, wherein the porous membrane defines a conjugate pad, the conjugate pad including the detection probes;

    a detection zone positioned downstream of the conjugate pad within which is immobilized a capture reagent that is configured to bind to the analyte complex to generate a detection signal; and

    a calibration zone positioned downstream of the detection zone within which a capture reagent is immobilized, the capture reagent being capable of binding to uncaptured detection probes to generate a calibration signal;

    ii) contacting the lateral flow assay device with the test sample;

    iii) subjecting the detection zone to illumination pulses to generate the detection signal;

    iv) subjecting the calibration zone to illumination pulses to generate the calibration signal; and

    v) thereafter, measuring the intensity of the detection signal and the calibration signal, wherein the amount of the analyte within the test sample is proportional to a ratio of the intensity of the detection signal to the intensity of the calibration signal.

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