Identifying antigen clusters for monitoring a global state of an immune system

• US 8,703,654 B2
• Filed: 08/11/2011
• Issued: 04/22/2014
• Est. Priority Date: 07/24/2000
• Status: Expired due to Fees

• Current Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Original Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Inventors: Cohen, Irun R., Domany, Eytan, Quintana, Francisco Javier, Hed, Guy, Getz, Gad
• Primary Examiner(s): Gross, Christopher M
• Application Number:
  US13/207,486
• Publication Number:
  US 20110301065A1
• Time in Patent Office: 985 Days
• Field of Search: None
• US Class Current: 506/18
• CPC Class Codes: G01N 33/564 for pre-existing immune complex or autoimmune disease , i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
• CPC Class Codes: G01N 33/6803 General methods of protein analysis not limited to specific proteins or families of proteins
• CPC Class Codes: G16B 20/00 ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
• CPC Class Codes: G16B 40/00 ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• CPC Class Codes: G16B 40/20 Supervised data analysis
• CPC Class Codes: G16B 40/30 Unsupervised data analysis

Abstract

Method, system and an article of manufacture for clustering and thereby identifying predefined antigens reactive with undetermined immunoglobulins of sera derived from patient subjects in need of diagnosis of disease or monitoring of treatment.

Claims

1. An article of manufacture comprising a surface and antigens being arranged on said surface, each antigen in an independent addressable location, wherein said antigens comprise:

Insulin and Aldolase each of cluster 5;

Cartilage extract of cluster 14;

Histone IIA, Lysine, Arginine, Laminin, Fibrinogen and Collagen VII each of cluster 26;

Collagen X of cluster 47; and

Cardiolipin of cluster 66.

2. The article of manufacture of claim 1, comprising an antigen chip.

3. The article of manufacture of claim 1, wherein said surface is selected from the group consisting of glass and PVDF membrane.

4. The article of manufacture of claim 1, wherein said antigens further comprise up to 25 additional antigens.

5. The article of manufacture of claim 1, wherein said antigens consist of:

Insulin and Aldolase each of cluster 5;

Cartilage extract of cluster 14;

Histone IIA, Lysine, Arginine, Laminin, Fibrinogen and Collagen VII each of cluster 26;

Collagen X of cluster 47; and

Cardiolipin of cluster 66.

6. The article of manufacture of claim 1 used for diagnosing Type I diabetes, wherein diagnosing Type I diabetes in a patient comprises:

determining an antibody pattern in a serum sample of the patient; and

identifying Type I diabetes in the patient if the antibody pattern comprises antibodies binding to the clustered antigens on the article of manufacture.