NMR analyzers for clinical evaluation of biosamples

• US 8,704,521 B2
• Filed: 08/11/2011
• Issued: 04/22/2014
• Est. Priority Date: 04/01/2004
• Status: Active Grant

• Current Assignee: LipoScience, Inc. (Laboratory Corporation Of America Holdings)
• Original Assignee: LipoScience, Inc. (Laboratory Corporation Of America Holdings)
• Inventors: Otvos, James D., Jeyarajah, Elias J., Markham, Stephen, Matyus, Steven P., Morgan, David R., Silberman, Bruce D., Deuel, Donald R.
• Primary Examiner(s): SHRIVASTAV, BRIJ B
• Application Number:
  US13/207,594
• Publication Number:
  US 20110295517A1
• Time in Patent Office: 985 Days
• Field of Search: 324322-300, 600407-445
• US Class Current: 324/318
• CPC Class Codes: G01R 33/307 specially adapted for moving the sample relative to the MR system, e.g. spinning mechanisms, flow cells or means for positioning the sample inside a spectrometer
• CPC Class Codes: G01R 33/465 applied to biological material, e.g. in vitro testing

Abstract

The clinical analyzers automatically electronically monitor selected parameters and automatically electronically adjust parameters to maintain the analyzer within desired operational ranges. The clinical NMR analyzers can be configured as a networked system with a plurality of clinical NMR analyzers located at different use sites.

Claims

1. A clinical NMR in vitro diagnostic analyzer, comprising:

an NMR spectroscopy instrument comprising a high-filed magnet with a magnet bore;

an automated sample handler configured to automatically introduce biosamples into the magnet bore;

means for automatically obtaining NMR signal spectra of the biosamples;

means for automatically electronically sensing data associated with selected operating parameters to verify that test conditions of the NMR diagnostic analyzer are within target operating ranges; and

means for automatically electronically adjusting selected operating parameters based on the verified test conditions.

2. A clinical NMR analyzer according to claim 1, further comprising:

means for automatically quantitatively and/or qualitatively analyzing the NMR signal spectra to obtain measurements of the respective biosamples; and

means for generating a patient report summarizing the measurements of the biosamples.

3. A clinical NMR analyzer according to claim 1, further comprising:

means for automatically monitoring NMR signal lineshapes of the obtained NMR signal spectra; and

means for automatically scaling the NMR signal spectra of the biosamples responsive to the monitored signal lineshape.

4. A clinical NMR analyzer according to claim 1, wherein the clinical NMR analyzer is configured to run an automated self-diagnostic quality control test at startup.

5. A clinical NMR analyzer according to claim 1, wherein the sample handler is configured to serially flow respective biosamples into an enclosed flow path that extends into the magnet bore, and wherein the analyzer comprises a non-transitory computer readable storage medium with computer program code that is configured to determine a patient'"'"'s risk of having and/or developing CHD based on the NMR derived quantitative measurements of the patient'"'"'s respective biosample.

6. A clinical NMR analyzer according to claim 1, wherein the clinical analyzer is configured to determine a patient'"'"'s risk of having and/or developing Type II diabetes based on the NMR derived quantitative measurements of the patient'"'"'s respective biosample.

7. A clinical NMR analyzer according to claim 1, wherein the analyzer include at least one processor configured to:

(i) obtain NMR spectra of a standard during operation;

(ii) perform a spectral deconvolution of a predetermined region of the obtained NMR spectra of the reference control material; and

(iii) compare data associated with the spectral deconvolution of the region with data associated with a priori spectra of the standard material to determine whether the NMR analyzer is in a suitable operational status and/or ready for diagnostic testing operation.

8. A clinical NMR analyzer according to claim 1, further comprising a top-loading flow probe that resides in the bore of the magnet to surround respective biosamples.

9. A clinical NMR analyzer according to claim 1, wherein the means for automatically obtaining, the means for automatically electronically sensing, and the means for automatically electronically adjusting include at least one electronic circuit in communication with an externally accessible user interface with at least one processor that carries out the obtaining, sensing and adjusting.

10. A clinical NMR analyzer according to claim 1, further comprising a single housing that encloses the sample handler and the NMR spectrometer and holds an externally accessible graphic user interface.

11. A clinical NMR in vitro diagnostic analyzer, comprising:

an NMR spectroscopy instrument with a high-field superconducting magnet having a magnet bore;

an automated sample handler configured to automatically introduce biosamples into the magnet bore;

a control circuit in electronic communication with the NMR spectroscopy instrument and sample handler; and

a plurality of electronic sensors disposed in the clinical NMR analyzer, the electronic sensors in communication with the control circuit, the electronic sensors configured to detect data associated with selected operating parameters to verify that selected conditions of the NMR diagnostic analyzer are within target operating ranges, wherein the control circuit is configured to automatically electronically adjust selected operating parameters and/or generate an alert based on data provided by the electronic sensors.

12. A clinical NMR analyzer according to claim 11, wherein the analyzer control circuit is configured to:

automatically electronically monitor NMR signal lineshapes of obtained NMR signal spectra of target biosamples; and

automatically electronically scale the NMR signal spectra of the biosamples responsive to the monitored signal lineshape.

13. A clinical NMR analyzer according to claim 11, wherein the clinical NMR analyzer is configured to run an automated electronic self-diagnostic quality control and/or calibration test at intervals during operation.

14. A clinical NMR analyzer according to claim 11, wherein the clinical NMR analyzer is configured as a single housing unit that encloses the NMR spectrometer and automated sample handler and holds an externally accessible graphic user interface.

15. A clinical NMR analyzer according to claim 11, further comprising a remote control circuit in communication with the clinical NMR analyzer, the remote control circuit and the clinical NMR analyzer configured to electronically communicate during operation whereby data regarding monitored operating parameters is transmitted to the remote control circuit.

16. A clinical NMR analyzer according to claim 15, in combination with a network of clinical NMR analyzers, the network of clinical analyzers being in communication with the remote control circuit, wherein the remote control circuit is configured to monitor selected local operating parameters associated with each clinical NMR analyzer.

17. A clinical NMR analyzer according to claim 11, wherein the control circuit is configured to automatically run a quality control status evaluation of selected operational parameters at intervals during operation.

18. A clinical NMR analyzer according to claim 11, further comprising a top-loading flow probe residing in the magnet bore that is in communication with the control circuit.

19. A clinical NMR analyzer according to claim 11, wherein the control circuit is configured to initiate an automated calibration procedure (i) at start-up as part of a self-diagnostic start-up procedure before authorizing or allowing evaluation of patient samples that are introduced to the flow probe held in the NMR spectrometer and (ii) when one or more of the selected parameters are determined to be outside desired operational ranges.

20. A clinical NMR analyzer according to claim 11, wherein the control circuit is configured to initiate a defined calibration procedure using a calibration standard delivered to a flow probe in the NMR spectrometer, and wherein the control circuit is configured to automatically abort a test, alert an operator of abnormal conditions and/or adjust operational parameters of selected components of the clinical NMR analyzer based on data obtained by the control circuit.

21. A clinical NMR in vitro diagnostic analyzer, comprising:

a housing;

an NMR spectrometer with a magnet having a bore held in the housing;

an automated sample handler held in the housing, the sample handler configured to automatically introduce biosamples into the magnet bore;

a plurality of sensors in the housing in communication with the NMR spectrometer; and

a control circuit in communication with the NMR spectrometer and sample handler, the control circuit configured to (i) automatically obtain NMR signal spectra of the biosamples;

(ii) automatically electronically monitor data from the sensors associated with selected operating parameters to verify that test conditions of the NMR diagnostic analyzer are within target operating ranges; and

(iii) automatically abort a test, alert an operator of abnormal conditions and/or adjust operational parameters of selected components of the clinical NMR analyzer.

22. A clinical NMR analyzer according to claim 21, wherein the control circuit includes at least one processor configured to automatically quantitatively and/or qualitatively analyze NMR signal spectra to obtain measurements of respective biosamples and generate respective patient reports summarizing the measurements of the biosamples.

23. A clinical NMR analyzer according to claim 21, wherein the automated sample handler comprises a sample handler interface and a sample injector interface, wherein the clinical NMR analyzer comprises an instrument automation module that electronically controls operation of the sample handler, the sample injector and the NMR spectrometer.

24. A clinical NMR analyzer according to claim 23, wherein the control circuit is configured to select what clinical quantitative measurement to be carried out for a respective biosample using the instrument automation module and a patient analytical test module, wherein the patient analytical test module includes a plurality of selectable different tests.

25. A clinical NMR analyzer according to claim 23, wherein the instrument automation module comprises an LIS interface.

26. A clinical NMR analyzer according to claim 23, wherein the NMR analyzer is configured to generate a customizable report format, one for each of a plurality of different test sites.

27. A clinical NMR analyzer according to claim 21, wherein the analyzer further comprises a flow cell held in a flow probe inside the magnet bore, wherein the sample handler holds containers of respective biosamples and the analyzer includes an enclosed flow path the directs the biosamples to flow into the flow cell held inside the flow probe in the magnet bore, and wherein the control circuit is configured to electronically direct an aspirating member to blow out an injection port associated with the flow path after injecting a first sample into the flow path before pre-fetching a second sample to maintain liquid-air gaps between neighboring samples in the flow path.