Cartridge for separating analyte from mixture, comprising dispensing and receiving chambers and insert

• US 8,709,358 B2
• Filed: 05/11/2011
• Issued: 04/29/2014
• Est. Priority Date: 05/26/2010
• Status: Active Grant

• Current Assignee: Maxaffinity LLC
• Original Assignee: Maxaffinity LLC
• Inventors: Liu, Xiao-Chuan
• Primary Examiner(s): Gakh, Yelena G
• Application Number:
  US13/105,736
• Publication Number:
  US 20110294224A1
• Time in Patent Office: 1,084 Days
• Field of Search: None
• US Class Current: 422/527
• CPC Class Codes: G01N 2021/0328 Arrangement of two or more cells having different functions for the measurement of reactions
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Devices, systems, and methods for separating an analyte from a mixture, and devices, systems, and methods for measuring an amount of an analyte are disclosed. The devices, systems, and methods may be used to separate glycated hemoglobin from other blood components and to measure the amount of the glycated hemoglobin.

Claims

1. A cartridge for separating an analyte from a mixture, the cartridge comprising:

two or more dispensing chambers at a top of the cartridge, each dispensing chamber having a dispensing aperture;

two or more receiving chambers at a bottom of the cartridge, each receiving chamber having a receiving aperture, the receiving chambers being positioned relative to the dispensing chambers so that the receiving chambers are capable of receiving the mixture from the dispensing chambers; and

an insert comprising a column comprising an affinity medium, the insert being configured to be positioned between at least one of the dispensing chambers and at least one of the receiving chambers to form a bottom of at least one of the dispensing apertures to block the at least one dispensing aperture,

wherein at least one of the dispensing chambers, the receiving chambers, or the insert is configured to move relative to the others to position the column comprising the affinity medium to be adjacent to one of the dispensing chambers to open the at least one dispensing aperture to form at least one dispensing flow path between the dispensing chamber and the affinity medium, and

wherein at least one of the dispensing chambers, the receiving chambers, or the insert is configured to move relative to the others to position the column comprising the affinity medium to be adjacent to one of the receiving chambers to open the at least one receiving aperture to form at least one receiving flow path between the affinity medium and the receiving chamber.

2. The cartridge of claim 1, wherein the insert is movably positioned between at least one of the dispensing chambers and at least one of the receiving chambers.

3. The cartridge of claim 2, wherein the cartridge is configured to selectively couple each of the dispensing chambers to the affinity medium according to the position of the insert.

4. The cartridge of claim 2, wherein the cartridge is configured to selectively couple the affinity medium to each of the receiving chambers according to the position of the insert.

5. The cartridge of claim 1, wherein the dispensing chambers and receiving chambers are movably positioned adjacent to the insert.

6. The cartridge of claim 5, wherein:

the cartridge is configured to selectively couple each of the dispensing chambers to the affinity medium according to the position of each of the dispensing chambers, and

the cartridge is configured to selectively couple the affinity medium to each of the receiving chambers according to the position of each of the receiving chambers.

7. The cartridge of claim 1, wherein:

the at least one dispensing flow path is configured to selectively couple one of the dispensing chambers to the affinity medium, and

the at least one receiving flow path is configured to selectively couple the affinity medium to one of the receiving chambers.

8. The cartridge of claim 1, wherein at least one of the dispensing chambers is configured to dispense a mixture comprising at least one reagent.

9. The cartridge of claim 1, wherein the at least one reagent is configured to lyse human blood cells.

10. The cartridge of claim 1, wherein at least one of the receiving chambers is configured to receive the analyte.

11. The cartridge of claim 1, wherein the analyte is glycated hemoglobin (hemoglobin A1c).

12. The cartridge of claim 1, wherein the affinity medium comprises a polymer comprising a binding moiety.

13. The cartridge of claim 12, wherein the polymer is cross-linked.

14. The cartridge of claim 12, wherein the polymer comprises agarose.

15. The cartridge of claim 12, wherein the polymer comprising a binding moiety is a reaction product of a polymer and a boronic acid or its derivative.

16. The cartridge of claim 15, wherein the polymer further comprises a spacer or an activator.

17. A system comprising the cartridge of claim 1, wherein the system is configured to measure the amount of the analyte.

18. The system of claim 17, wherein the system further comprises a spectrometer.