Avirulent oncolytic herpes simplex virus strains engineered to counter the innate host response
First Claim
1. A vaccine comprising:
- (a) a modified herpes simplex virus (HSV), and(b) a pharmaceutically acceptable vehicle for in situ administration to the subject,wherein the modified HSV has an intact Us12 gene and an endogenous Us11 gene expressed as a late gene, and is modified from a wild-type HSV with both γ
134.5 genes of the virus being deleted and Us11 genes that are expressed as intermediate early (IE) genes being inserted in the γ
134.5 gene locus in place of both γ
134.5 genes.
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Accused Products
Abstract
The present invention relates to an avirulent, oncolytic herpes simplex virus modified from a wild-type herpes simplex virus so that both γ134.5 genes of the virus have been deleted and each replaced with an interferon-resistance gene that is expressed as an immediate-early gene. The present invention also relates to a pharmaceutical composition that includes the modified herpes simplex virus of the present invention and a pharmaceutically acceptable vehicle for in situ administration to tumor cells. Also provided in the present invention are methods for killing tumor cells in a subject and for immunizing a subject against an infectious disease, cancer, or an autoimmune disease that involve administering to a subject the modified avirulent, oncolytic herpes simplex virus of the present invention.
48 Citations
12 Claims
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1. A vaccine comprising:
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(a) a modified herpes simplex virus (HSV), and (b) a pharmaceutically acceptable vehicle for in situ administration to the subject, wherein the modified HSV has an intact Us12 gene and an endogenous Us11 gene expressed as a late gene, and is modified from a wild-type HSV with both γ
134.5 genes of the virus being deleted and Us11 genes that are expressed as intermediate early (IE) genes being inserted in the γ
134.5 gene locus in place of both γ
134.5 genes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification