Microfluidic cartridge and method of using same
DCFirst Claim
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1. A microfluidic cartridge comprising a microfluidic substrate layer, the microfluidic substrate layer comprising:
- a first reaction chamber;
a second reaction chamber;
a first inlet port for introducing a first sample onto the microfluidic substrate layer, the first inlet port formed in a surface of the microfluidic substrate layer and in fluid communication with the first reaction chamber;
a second inlet port for introducing a second sample onto the microfluidic substrate layer, the second inlet port spaced apart from the first inlet port on the surface of the microfluidic substrate layer, the second inlet port in fluid communication with the second reaction chamber;
a first outlet, in fluid communication with the first reaction chamber;
a second outlet, in fluid communication with the second reaction chamber;
a first set of microfluidic valves configured to isolate the first reaction chamber from the first inlet port and the first outlet; and
a second set of microfluidic valves configured to isolate the second reaction chamber from the second inlet port and the second outlet independent of the isolation of the first reaction chamber by the first set of microfluidic valves,wherein the isolation effected by the first and the second set of microfluidic valves prevents movement of fluid into and out of the first and the second reaction chambers, wherein the first set of microfluidic valves comprises a first microfluidic valve spatially separated from the first inlet port and a second microfluidic valve spatially separated from the first outlet, and wherein the second set of microfluidic valves comprises a first microfluidic valve spatially separated from the second inlet port and a second microfluidic valve spatially separated from the second outlet, and wherein each of the first and second reaction chambers, the first and second inlet ports, the first and second outlets, and the first and second sets of microfluidic valves are all formed in the microfluidic substrate layer.
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Abstract
The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
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Citations
22 Claims
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1. A microfluidic cartridge comprising a microfluidic substrate layer, the microfluidic substrate layer comprising:
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a first reaction chamber; a second reaction chamber; a first inlet port for introducing a first sample onto the microfluidic substrate layer, the first inlet port formed in a surface of the microfluidic substrate layer and in fluid communication with the first reaction chamber; a second inlet port for introducing a second sample onto the microfluidic substrate layer, the second inlet port spaced apart from the first inlet port on the surface of the microfluidic substrate layer, the second inlet port in fluid communication with the second reaction chamber; a first outlet, in fluid communication with the first reaction chamber; a second outlet, in fluid communication with the second reaction chamber; a first set of microfluidic valves configured to isolate the first reaction chamber from the first inlet port and the first outlet; and a second set of microfluidic valves configured to isolate the second reaction chamber from the second inlet port and the second outlet independent of the isolation of the first reaction chamber by the first set of microfluidic valves, wherein the isolation effected by the first and the second set of microfluidic valves prevents movement of fluid into and out of the first and the second reaction chambers, wherein the first set of microfluidic valves comprises a first microfluidic valve spatially separated from the first inlet port and a second microfluidic valve spatially separated from the first outlet, and wherein the second set of microfluidic valves comprises a first microfluidic valve spatially separated from the second inlet port and a second microfluidic valve spatially separated from the second outlet, and wherein each of the first and second reaction chambers, the first and second inlet ports, the first and second outlets, and the first and second sets of microfluidic valves are all formed in the microfluidic substrate layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A microfluidic substrate, comprising:
a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another; an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent, wherein the first valve and the second valve are configured to isolate the reaction chamber from the inlet and the vent to prevent movement of fluid into or out of the reaction chamber, wherein the first valve is spatially separated from the inlet and the second valve is spatially separated from the vent, wherein the reaction chamber, the first channel, and the second channel are formed in a first side of the microfluidic substrate, wherein the inlet and the vent are formed in a second side of the microfluidic substrate opposite the first side, and wherein the first valve in each of the plurality of sample lanes is operated independently of any other first valve. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
Specification