Biological analysis arrangement and approach therefor
First Claim
1. An apparatus comprising:
- an integrated circuit chip configured and arranged as a one-sided biological sensing architecture that includesa substrate of the integrated circuit chip having a first side;
a plurality of test sites that are recessed into the first side of the integrated circuit chip substrate and configured and arranged in a planar array;
a plurality of photodiodes that are integrated into the recesses of the plurality of test sites and that are configured and arranged with a diffusion doping region having a dopant concentration and junction depth sufficient to detect firefly luciferase light produced during a pyrosequencing reaction within the plurality of test sites and generated from a sample contained within the test sites;
an analog to digital converter circuit fabricated as part of the integrated circuit chip and configured and arranged to convert analog voltages from the plurality of photodiodes into digital values; and
a memory circuit configured and arranged to store the digital values and that is fabricated as part of the integrated circuit chip.
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Accused Products
Abstract
Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample.
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Citations
18 Claims
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1. An apparatus comprising:
an integrated circuit chip configured and arranged as a one-sided biological sensing architecture that includes a substrate of the integrated circuit chip having a first side; a plurality of test sites that are recessed into the first side of the integrated circuit chip substrate and configured and arranged in a planar array; a plurality of photodiodes that are integrated into the recesses of the plurality of test sites and that are configured and arranged with a diffusion doping region having a dopant concentration and junction depth sufficient to detect firefly luciferase light produced during a pyrosequencing reaction within the plurality of test sites and generated from a sample contained within the test sites; an analog to digital converter circuit fabricated as part of the integrated circuit chip and configured and arranged to convert analog voltages from the plurality of photodiodes into digital values; and a memory circuit configured and arranged to store the digital values and that is fabricated as part of the integrated circuit chip. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. An apparatus comprising:
an integrated circuit chip configured and arranged as a one-sided biological sensing architecture that includes a substrate of the integrated circuit chip having a first side; a plurality of test sites that are recessed into the first side of the integrated circuit chip substrate and configured and arranged in a planar array; a plurality of photodiodes that are integrated into the recesses of the plurality of test sites and that are configured and arranged with a diffusion doping region having a dopant concentration and junction depth sufficient to detect firefly luciferase light produced during a pryosequencing reaction within the plurality of test sites and generated from a sample contained within the test sites; an analog to digital converter circuit fabricated as part of the integrated circuit chip and configured and arranged to convert analog voltages from the plurality of photodiodes into digital values; a memory circuit configured and arranged to store the digital values and that is fabricated as part of integrated circuit chip; and wherein the substrate is a P-type substrate with a dopant concentration of about 1015 dopant species per cm3 and wherein the plurality of photodiodes each include an N-type diffusion with a dopant concentration of about 5*1015 dopant species per cm3.
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15. A method of detecting luciferase light generation, the method comprising:
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providing a reagent to a plurality of test sites that are recessed into a first side of an integrated circuit chip substrate and that are arranged in a planar array; measuring charge accumulation in a plurality of photodiodes that are integrated into the recesses of the plurality of test sites and that are configured and arranged with a diffusion doping region having a dopant concentration and junction depth sufficient to detect firefly luciferase light produced during a pyrosequencing reaction within the plurality of test sites and generated responsive to interaction between the reagent and test samples contained within the plurality of test sites; converting the measured charge accumulation to a digital value using an analog to digital converter circuit that is fabricated as part of the integrated circuit chip substrate; and storing the digital values in a memory circuit that is fabricated as part of the integrated circuit chip substrate. - View Dependent Claims (16, 17, 18)
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Specification