Human embryonic stem cell methods and PODXL expression

• US 8,710,190 B2
• Filed: 04/12/2012
• Issued: 04/29/2014
• Est. Priority Date: 03/06/2006
• Status: Expired due to Fees

• Current Assignee: Agency For Science Technology and Research (Government of Singapore)
• Original Assignee: Agency For Science Technology and Research (Government of Singapore)
• Inventors: Choo, Andre, Oh, Steve
• Primary Examiner(s): BELYAVSKYI, MICHAIL A
• Application Number:
  US13/445,705
• Publication Number:
  US 20120219962A1
• Time in Patent Office: 747 Days
• Field of Search: None
• US Class Current: 530/387.9
• CPC Class Codes: A61K 35/545 Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: G01N 33/5073 Stem cells

Abstract

A method of identifying an undifferentiated human embryonic stem cell in a sample which may contain such cells, the method comprising identifying the cell or cells within the sample that express podocalyxin-like protein (PODXL) on their surface. A method of isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface. Typically, the methods use an antibody which binds to PODXL. Undifferentiated human embryonic stem cells isolated by the method may be useful in cell therapy. Also, in particular, compositions of cells differentiated from a human embryonic stem cell but which composition has been depleted of undifferentiated human embryonic stem cells are provided which are useful in cell therapy.

Claims

1. A kit comprising an antibody that specifically binds PODXL on the surface of a cell, and which is cytotoxic to said cell, and an agent that detects a human embryonic stem cell marker, wherein the antibody comprises

(a) at least one light chain variable region comprising the following complementarity determining regions (CDRs):

2. The kit according to claim 1 wherein the human embryonic stem cell marker is selected from the group consisting of Oct 4, SSEA-4, Tra-1-60, Tra-1-81 and GCTM-2.

3. The kit according to claim 1, comprising at least 5 μ

g of the antibody.

4. The kit according to claim 1 wherein the antibody has at least one light chain variable region comprising the amino acid sequence of SEQ ID NO:

8.

5. The kit according to claim 1 wherein the antibody has at least one heavy chain variable region comprising the amino acid sequence of SEQ ID NO:

10.