Nucleic acid hydrogel via rolling circle amplification
First Claim
1. A composition comprising a nucleic acid hydrogel, the nucleic acid hydrogel comprising entangled single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded entangled nucleic acid molecules are formed via rolling cycle amplification, and further wherein said nucleic acid hydrogel has a matrix for structural support, wherein said matrix is formed by gelation of said single-stranded entangled nucleic acid molecules and water, wherein the gelation occurs from a mixture consisting essentially of at least one single-stranded entangled nucleic acid molecule and water.
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Abstract
Methods and compositions are provided for producing nucleic acid-based compositions. Methods include enzyme catalyzed or nucleic acid polymer conjugation. Compositions include nucleic acid-containing hydrogels which can be elongated via rolling circle amplification. The hydrogels can encapsulate bioactive agents for drug delivery.
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Citations
28 Claims
- 1. A composition comprising a nucleic acid hydrogel, the nucleic acid hydrogel comprising entangled single-stranded nucleic acid molecules, wherein at least a portion of the single-stranded entangled nucleic acid molecules are formed via rolling cycle amplification, and further wherein said nucleic acid hydrogel has a matrix for structural support, wherein said matrix is formed by gelation of said single-stranded entangled nucleic acid molecules and water, wherein the gelation occurs from a mixture consisting essentially of at least one single-stranded entangled nucleic acid molecule and water.
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2. A composition comprising a nucleic acid hydrogel, the nucleic acid hydrogel comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second single-stranded nucleic acid molecule, the second single-stranded nucleic acid molecule formed from the amplification of the first single-stranded nucleic acid molecule using a second primer, and further wherein said nucleic acid hydrogel has a matrix for structural support, wherein said matrix is formed by gelation of said single-stranded nucleic acid molecules and water, wherein the gelation occurs from a mixture consisting essentially of at least one first single-stranded nucleic acid molecule, at least one second single-stranded nucleic acid molecule, and water. - View Dependent Claims (7, 8, 9, 10, 11)
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3. A composition comprising a nucleic acid hydrogel, the nucleic acid hydrogel comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second single-stranded nucleic acid molecule, the second single-stranded nucleic acid molecule formed from the amplification of the first single-stranded nucleic acid molecule using a second primer, and further comprising a third single-stranded nucleic acid molecule, the third single-stranded nucleic acid molecule formed from the amplification of the second single-stranded nucleic acid molecule using a third primer, and further wherein said nucleic acid hydrogel has a matrix for structural support, wherein said matrix is formed by gelation of at least one first single-stranded nucleic acid molecule, at least one second single-stranded nucleic acid molecule, at least one third single-stranded nucleic acid molecule, and water, wherein the gelation occurs from a mixture consisting essentially of at least one first single-stranded nucleic acid molecule, at least one second single-stranded nucleic acid molecule, at least one third single-stranded nucleic acid molecule, and water. - View Dependent Claims (4, 5, 6)
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12. A composition comprising a nucleic acid hydrogel, the nucleic acid hydrogel comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second nucleic acid molecule, and further wherein said nucleic acid hydrogel has a matrix for structural support, wherein the matrix is formed by gelation of said single-stranded nucleic acid molecules and water, wherein the gelation occurs from a mixture consisting essentially of at least one first single-stranded nucleic acid molecule, at least one second nucleic acid molecule, and water. - View Dependent Claims (13, 14)
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15. A nucleic acid hydrogel, comprising:
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a first single-stranded nucleic acid molecule, the first single-stranded nucleic acid molecule formed from the amplification of a circular nucleic acid template using a first primer; and a second nucleic acid molecule; wherein the first nucleic acid molecule comprises DNA and the second nucleic acid molecule comprises RNA. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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Specification