Delignification of biomass with sequential base treatment
First Claim
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1. A method for the production of a saccharification product, the method comprising:
- a) providing a cellulosic feedstock comprising lignin;
b) contacting, in water, the feedstock of (a) with at least one metal carbonate at a time of 0 hour at a temperature of no more than 200°
C. to produce a first delignification slurry;
c) contacting the first delignification slurry with a nucleophilic base at a concentration of no more than 8% on a mass basis at a time of at least 0+0.5 hour and at a temperature of no more than 200°
C. to form a second delignification slurry comprising a lignin-containing liquid fraction and a polysaccharide-enriched solid fraction;
d) separating the lignin-containing liquid fraction from the polysaccharide-enriched solid fraction; and
e) contacting at least a portion of the polysaccharide-enriched solid fraction with an enzyme consortium to produce a saccharification product comprising xylose and glucose monomers wherein the overall yield of xylose and glucose monomers in the saccharification product are individually at least about 50%;
wherein said enzyme consortium consists essentially of cellulases and xylanases and wherein saccharification occurs under the conditions of a pH of about 4.5 to about 5.7 and a temperature of about 45°
C. to about 55°
C.
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Abstract
A method for the production of a saccharification product is provided, the method comprising:
- a) providing a cellulosic feedstock comprising lignin;
- b) contacting, in water, the feedstock of (a) with at least one metal carbonate at time 0 hour under suitable initial reaction conditions to produce a first delignification slurry;
- c) contacting the first delignification slurry with a nucleophilic base at time (0+n) hour, where n represents a number greater than zero, under suitable secondary reaction conditions to form a second delignification slurry comprising a lignin-containing liquid fraction and a polysaccharide-enriched solid fraction;
- d) separating the lignin-containing liquid fraction from the polysaccharide-enriched solid fraction; and
- e) contacting at least a portion of the polysaccharide-enriched solid fraction with an enzyme consortium to produce a saccharification product comprising xylose and glucose monomers wherein the overall yield of xylose and glucose monomers in the saccharification product are individually at least about 50%.
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Citations
11 Claims
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1. A method for the production of a saccharification product, the method comprising:
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a) providing a cellulosic feedstock comprising lignin; b) contacting, in water, the feedstock of (a) with at least one metal carbonate at a time of 0 hour at a temperature of no more than 200°
C. to produce a first delignification slurry;c) contacting the first delignification slurry with a nucleophilic base at a concentration of no more than 8% on a mass basis at a time of at least 0+0.5 hour and at a temperature of no more than 200°
C. to form a second delignification slurry comprising a lignin-containing liquid fraction and a polysaccharide-enriched solid fraction;d) separating the lignin-containing liquid fraction from the polysaccharide-enriched solid fraction; and e) contacting at least a portion of the polysaccharide-enriched solid fraction with an enzyme consortium to produce a saccharification product comprising xylose and glucose monomers wherein the overall yield of xylose and glucose monomers in the saccharification product are individually at least about 50%;
wherein said enzyme consortium consists essentially of cellulases and xylanases and wherein saccharification occurs under the conditions of a pH of about 4.5 to about 5.7 and a temperature of about 45°
C. to about 55°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification