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Manipulation of cells on a droplet actuator

  • US 8,716,015 B2
  • Filed: 12/15/2008
  • Issued: 05/06/2014
  • Est. Priority Date: 04/18/2006
  • Status: Expired due to Fees
First Claim
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1. A method of inoculating and maintaining a culture medium, the method comprising:

  • (a) providing a droplet actuator comprising a sample droplet loaded thereon, wherein the sample droplet is substantially surrounded by an oil filler fluid and comprises cells of multiple cell types;

    (b) dispensing by electrowetting a sub-droplet from the sample droplet;

    (c) analyzing the sub-droplet to determine whether the sub-droplet comprises a single cell type;

    (d) repeating steps (b) and (c) until one or more sub-droplets each comprising a single cell type is identified; and

    (e) inoculating a culture medium droplet on the droplet actuator by executing electrowetting-mediated droplet operations with a sub-droplet of (c) or (d) comprising a single cell type to bring the sub-droplet into contact with the culture medium droplet to form an inoculated droplet; and

    (f) maintaining the inoculated droplet of (e) at a temperature suitable for causing the cells to grow in the inoculated droplet surrounded by the oil on the droplet actuator, and thereby causing the cells to grow.

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