Granulocyte colony stimulating factor: remodeling and glycoconjugation G-CSF

• US 8,716,239 B2
• Filed: 01/11/2007
• Issued: 05/06/2014
• Est. Priority Date: 10/10/2001
• Status: Active Grant

• Current Assignee: Novo Nordisk A/S
• Original Assignee: Novo Nordisk A/S
• Inventors: DeFrees, Shawn, Zopf, David A., Bayer, Robert J., Bowe, Caryn, Hakes, David James, Chen, Xi
• Primary Examiner(s): GOON, SCARLETT Y
• Application Number:
  US11/652,467
• Publication Number:
  US 20080050772A1
• Time in Patent Office: 2,672 Days
• Field of Search: 514/8, 514/20.9
• US Class Current: 514/20.9
• CPC Class Codes: C07K 1/006 of peptides containing derivatised side chain amino acids
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
• CPC Class Codes: C07K 1/13 Labelling of peptides
• CPC Class Codes: C07K 14/505 Erythropoietin [EPO]
• CPC Class Codes: C07K 14/535 Granulocyte CSF; Granulocyte-macrophage CSF
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/91 containing a motif for glycosylation
• CPC Class Codes: C07K 9/00 Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 21/005 Glycopeptides, glycoproteins

Abstract

The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.

Claims

1. A granulocyte-colony stimulating factor peptide conjugate having the formula

2. The conjugate of claim 1, wherein the water-soluble polymer is polyethylene glycol.

3. A composition comprising the conjugate of claim 1 and a carrier therefor.

4. A composition comprising the conjugate of claim 2 and a carrier therefor.