Cottonseed oil and uses

• US 8,716,555 B2
• Filed: 01/21/2011
• Issued: 05/06/2014
• Est. Priority Date: 07/21/2008
• Status: Active Grant

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Inventors: Liu, Qing, Green, Allan Graham, Singh, Surinder Pal
• Primary Examiner(s): Page, Brent T
• Assistant Examiner(s): KEOGH, MATTHEW R
• Application Number:
  US13/011,773
• Publication Number:
  US 20110223311A1
• Time in Patent Office: 1,201 Days
• Field of Search: None
• US Class Current: 800/281
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 10/37 from waste material from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: C07H 13/06 Fatty acids
• CPC Class Codes: C12N 15/1137 against enzymes viral enzymes C12N15/1131; receptors C12N15/1138
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8243 involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
• CPC Class Codes: C12N 2310/11 Antisense
• CPC Class Codes: C12N 2310/12 catalytic nucleic acids, e.g. ribozymes
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12N 5/14 Plant cells
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12Y 114/19006 : DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase
• CPC Class Codes: C12Y 203/01085 : Fatty-acid synthase (2.3.1.85)
• CPC Class Codes: C12Y 301/02014 : Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
• CPC Class Codes: D01B 1/04 : Ginning
• CPC Class Codes: Y02P 60/87 : Re-use of by-products of food processing for fodder production

Abstract

The present specification relates to the production of cotton plants and seeds and oil prepared therefrom having elevated levels of oleic and reduced levels of palmitic and linoleic acids. Furthermore, cottonseeds having low levels of cyclopropane and/or cyclopropene fatty acids and/or reduced levels of gossypol are described herein. The specification also describe FatB and CPA-FAS nucleotide and amino acid sequences derived from cotton facilitating, inter alia the direct modification of plant oil content and/or composition.

Claims

1. A process for producing cottonseed oil comprising the steps of

(i) obtaining cottonseed of the species Gossypium hirsutum, wherein in the oil of the cottonseed 72% to 88% by weight of the total fatty acid content is oleic acid, 4% to 16% by weight of the total fatty acid content is palmitic acid and 4% to 15% by weight of the total fatty acid content is linoleic acid, and wherein the cottonseed has a reduced level of each of cotton FAD2-1 protein, cotton FatB-2 protein, and cotton CPA-FAS-2 protein relative to the level of cotton FAD2-1 protein, cotton FatB-2 protein, and cotton CPA-FAS-2 protein, respectively, in cottonseed of cotton variety Coker when grown under the same conditions;

(ii) extracting the oil from the cottonseed; and

(iii) recovering the cottonseed oil.

2. The process of claim 1, wherein 0.1% to 0.5% by weight of the total fatty acid content in the cottonseed oil is cyclopropane fatty acid (CPA), cyclopropene fatty acid (CPE) or a combination of CPA and CPE.

3. The process of claim 2, wherein the fatty acid of the CPA or the CPE is malvalic acid, sterculic acid, dihydrosterculic acid or any combination of two or all three of these fatty acids.

4. The process of claim 1, wherein the level of gossypol in the cottonseed is reduced by at least 10% relative to the level of gossypol in cottonseed of cotton variety Coker.

5. The process of claim 2, wherein the level of gossypol in the cottonseed is reduced by at least 10% relative to the level of gossypol in cottonseed of cotton variety Coker.

6. The process of claim 1, wherein step (ii) comprises crushing the cottonseed.

7. The process of claim 1, wherein step (iii) comprises purifying the cottonseed oil.

8. The process of claim 1, wherein the cottonseed oil is not hydrogenated.

12. The process of claim 1, wherein 4% to 10% by weight of the total fatty acid content in the cottonseed oil is palmitic acid.

14. The process of claim 1, wherein the cottonseed has a germination rate which is substantially the same as cottonseed of the cotton variety Coker.

9. A cottonseed of the species Gossypium hirsutum comprising oil, wherein in the oil of the cottonseed 72% to 88% by weight of the total fatty acid content is oleic acid, 4% to 16% by weight of the total fatty acid content is palmitic acid and 4% to 15% by weight of the total fatty acid content is linoleic acid, and wherein the cottonseed has a reduced level of each of cotton FAD2-1 protein, cotton FatB-2 protein and cotton CPA-FAS-2 protein relative to the levels of cotton FAD2-1 protein, cotton FatB-2 protein and cotton CPA-FAS-2 protein respectively, in cottonseed of cotton variety Coker when grown under the same conditions.

10. The cottonseed of claim 9, wherein 0.1% to 0.5% by weight of the total fatty acid content in the cottonseed oil is cyclopropane fatty acid (CPA), cyclopropene fatty acid (CPE) or a combination of CPA and CPE.

11. The cottonseed of claim 9, wherein the level of gossypol in the cottonseed is reduced by at least 10% relative to the level of gossypol in cottonseed of cotton variety Coker.

13. The cottonseed of claim 9, wherein 4% to 10% by weight of the total fatty acid content in the cottonseed oil is palmitic acid.

15. The cottonseed of claim 9, wherein the cottonseed has a germination rate which is substantially the same as cottonseed of the cotton variety Coker.

16. The cottonseed of claim 9, wherein

i) 0.1% to 0.5% by weight of the total fatty acid content in the cottonseed oil is cyclopropane fatty acid (CPA), cyclopropene fatty acid (CPE) or a combination of CPA and CPE, and

ii) the level of gossypol in the cottonseed is reduced by at least 10% relative to the level of gossypol in cottonseed of cotton variety Coker.