Membrane system with sufficient buffering capacity
First Claim
Patent Images
1. An electrochemical analyte sensor comprising:
- at least one electrode having an electroactive surface;
an analyte sensing membrane, at least a portion of the membrane covering the electroactive surface, the membrane comprising;
an optional interference layer;
an enzyme layer;
an optional hydrophilic polymer layer positioned (i) between the electroactive surface and the optional interference layer and/or (ii) between the optional interference layer and the enzyme layer; and
at least one buffering agent associated with the analyte sensing membrane, wherein the buffering agent comprises a polycation salt, the polycation salt comprising at least one of acetate ion, carbonate ion, bicarbonate ion, or combinations thereof.
1 Assignment
0 Petitions
Accused Products
Abstract
Electrochemical sensors for measurement of an analyte comprising an analyte sensing membrane comprising at least one salt of acetate ion, carbonate ion, bicarbonate ion, or mixtures thereof. Sensor testing methods comprising contacting an electrochemical sensor with an aqueous solution comprising at least one salt of acetate ion, carbonate ion, bicarbonate ion, or mixtures thereof and contacting the electrochemical sensor with one or more concentrations of analyte, the one or more concentrations of analyte being in the clinical concentration range of the analyte.
-
Citations
22 Claims
-
1. An electrochemical analyte sensor comprising:
-
at least one electrode having an electroactive surface; an analyte sensing membrane, at least a portion of the membrane covering the electroactive surface, the membrane comprising; an optional interference layer; an enzyme layer; an optional hydrophilic polymer layer positioned (i) between the electroactive surface and the optional interference layer and/or (ii) between the optional interference layer and the enzyme layer; and at least one buffering agent associated with the analyte sensing membrane, wherein the buffering agent comprises a polycation salt, the polycation salt comprising at least one of acetate ion, carbonate ion, bicarbonate ion, or combinations thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. An electrochemical analyte sensor comprising:
-
at least one working electrode having an electroactive surface; an analyte sensing membrane comprising; a hydrophilic layer; an optional interference layer; an enzyme layer; and at least one buffering agent consisting of a polycation salt of any one of acetate ion, carbonate ion, bicarbonate ion, or mixtures thereof, the at least one buffering agent being;
(i) a dried layer between the electroactive surface and the optional interference layer;
(ii) a dried layer between the optional interference layer and the enzyme layer;
(iii) associated with the optional hydrophilic polymer layer, the hydrophilic layer positioned (a) between the electrode surface and the optional interference layer and/or (b) between the optional interference layer and the enzyme layer;
(iv) associated with the enzyme layer;
or (v) any or all combinations of (i)-(iv);wherein the hydrophilic layer is positioned (i) between the electroactive surface and the optional interference layer or enzyme layer and/or (ii) between the optional interference layer and an enzyme layer, the hydrophilic layer comprising at least one hydrophilic polymer and excluding an enzyme. - View Dependent Claims (13, 14, 15, 16, 17, 18)
-
-
19. An in vitro, electrochemical analyte sensor testing method, the method comprising:
-
providing an electrochemical sensor; contacting the electrochemical sensor to an aqueous solution comprising a buffering agent, wherein the buffering agent comprises acetate ion, carbonate ion, bicarbonate ion, phosphate buffered saline (PBS) of at least 0.030 molality Na2HPO4 and at least 0.006 molality KH2PO4, or any combination of acetate ion, carbonate ion, bicarbonate ion, and phosphate buffered saline (PBS), the buffering agent providing a signal drift at 400 mg/dL glucose or more of 10% or less over a time period of at least one hour; contacting the electrochemical sensor with one or more concentrations of analyte, the one or more concentrations of analyte being in the clinical concentration range of the analyte; and testing the electrochemical analyte sensor. - View Dependent Claims (20, 21, 22)
-
Specification