Genes for enhanced lipid metabolism for accumulation of lipids

• US 8,722,359 B2
• Filed: 01/21/2011
• Issued: 05/13/2014
• Est. Priority Date: 01/21/2011
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Kilian, Oliver, Vick, Bertrand
• Primary Examiner(s): Page, Brent T
• Assistant Examiner(s): KEOGH, MATTHEW R
• Application Number:
  US13/011,809
• Publication Number:
  US 20120190115A1
• Time in Patent Office: 1,208 Days
• Field of Search: None
• US Class Current: 435/41
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12Y 203/0102 Diacylglycerol O-acyltransferase (2.3.1.20)

Abstract

Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene.

Claims

1. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 1.

2. The method of claim 1, wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.

3. The method of claim 1, wherein the algal cell is of algal genus Nannochloropsis.

4. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 2.

5. The method of claim 4, wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.

6. The method of claim 4, wherein the algal cell is of algal genus Nannochloropsis.

7. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequence set forth in SEQ. ID. NO. 3.

8. The method of claim 7, wherein a promoter in an expression vector increases expression of the nucleotide sequence compared to a wild-type algal cell.

9. The method of claim 7, wherein the algal cell is of algal genus Nannochloropsis.

10. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1 and SEQ. ID. NO. 2.

11. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1 and SEQ. ID. NO. 3.

12. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 2 and SEQ. ID. NO. 3.

13. A method for increasing lipid accumulation compared to a wild-type algal cell, the method comprising transforming an algal cell with the nucleotide sequences set forth in SEQ. ID. NO. 1, SEQ. ID. NO. 2, and SEQ. ID. NO. 3.