Chromatography quantitative measuring apparatus

• US 8,722,425 B2
• Filed: 12/18/2009
• Issued: 05/13/2014
• Est. Priority Date: 09/25/2000
• Status: Expired due to Fees

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Miyoshi, Koji, Aga, Masahiro, Shigematsu, Kaoru
• Primary Examiner(s): Chin, Chris L
• Application Number:
  US12/641,903
• Publication Number:
  US 20100104475A1
• Time in Patent Office: 1,607 Days
• Field of Search: None
• US Class Current: 436/514
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 30/95 Detectors specially adapted therefor; Signal analysis
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: Y10S 435/967 Standards, controls, materials, e.g. validation studies, buffer systems
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

In a chromatography quantitative measuring apparatus according to the present invention, a beam applied from a light source to a chromatography test strip is formed into an elliptical shape by an optical means such as a cylindrical lens, a variation in absorbance that accompanies elution of a marker regent is detected while the elliptical beam is applied between a marker reagent hold part and a detection part, and a measurement is automatically started in a prescribed period of time since the detection of variation. According to the chromatography quantitative measuring apparatus so configured, non-uniform coloration is reduced by shaping the beam elliptically with the optical means, whereby the accuracy of quantitative analysis is enhanced, and the apparatus can be operated easily.

Claims

1. A chromatography quantitative measuring method for a chromatography test strip, the chromatography test strip including an application part where a blood sample is applied, a marker reagent hold part which holds a marker reagent that can be eluted by development of the blood sample, a base part where a specific binding reaction is caused between the marker reagent and an analysis target included in the blood sample, and a detection part where a bounded material of the marker reagent and the analysis target is immobilized, said method comprising:

applying an optical beam emitted from a light source to the base part of the chromatography test strip,

detecting an optical signal utilizing transmitted light or reflected light from the base part of the chromatography test strip,

determining whether the blood sample is a plasma sample or a whole blood sample from the optical signal detected from the base part on the chromatography test strip where the blood sample is applied and development is completed,

selecting a calibration curve that conforms to the blood sample determined, and

measuring the concentration of the analysis target by using the selected calibration curve corresponding to the determined kind of the blood sample.

2. The chromatography quantitative measuring method as defined in claim 1, wherein

the optical signal is detected downstream of the detection part in the direction of the development of the blood sample.